Antibodies for bioanalysis and drug monitoring of golimumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug golimumab (Simponi) using our range of ready-made anti-idiotypic antibodies.

  • Highly specific to golimumab or the golimumab-TNFα complex
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Golimumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Golimumab Inhibitory

Type 1

HCA240

AbD20710

Fab-FH1

2.3

PK bridging ELISA

Order HCA240

HCA241

AbD20692_hIgG1

Human

IgG1

2.8

PK bridging ELISA

ADA control

Order HCA241

HCA286

AbD254292

Fab-FH1

1.0

PK bridging ELISA

Order HCA286

HCA287
HCA287P

AbD25418_hIgG12

Human IgG1

HRP

0.1

PK bridging ELISA

ADA control

Order HCA287
Order HCA287P

Golimumab & Golimumab-TNFα

Non-Inhibitory

Type 2

HCA242

AbD20897

Fab-FH1

7

PK bridging ELISA

Order HCA242

HCA243

AbD20897_hIgG1

Human IgG1

7

PK bridging ELISA

Order HCA243

HCA289

AbD254552

Fab-FH1

0.9

PK bridging ELISA

Order HCA289

HCA290
HCA290P

AbD25451_hIgG12

Human IgG1

HRP

0.4

PK bridging ELISA

Order HCA290
Order HCA290P

Golimumab-TNFα Complex Specific

Type 3

HCA274

AbD257052

Fab-FH1

6

PK ELISA antigen capture format

Order HCA274

HCA245

AbD20893_hIgG1

Human IgG1

53

PK ELISA antigen capture format

Order HCA245

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags
2 Affinity matured antibodies: HCA286 is derived from AbD20710 (HCA240); HCA287 is derived from AbD20692 (HCA241); HCA289 and HCA290 are derived from AbD20897 (HCA242); HCA274 is derived from AbD20893 (HCA245)


Anti-Golimumab Inhibitory Antibodies (Type 1)

Type 1 anti-golimumab antibodies inhibit the binding of the drug golimumab to its target, tumor necrosis factor alpha (TNFα). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.

Anti-Golimumab Non-Inhibitory Antibodies (Type 2)

The type 2 anti-golimumab antibodies bind to the golimumab idiotope, but do not inhibit the binding of golimumab to TNFα. They can therefore be used in a PK bridging ELISA to measure total drug - free, partially bound and fully bound.

Anti-Golimumab-TNFα Complex Specific Antibody (Type 3)

The Type 3 antibodies specifically recognize the drug-target complex, detecting golimumab only when it is bound to TNFα. They are suitable as a detection antibody in a PK antigen capture assay.


ELISA Protocols to Get You Started

PK Bridging ELISA

Fig. 1. Golimumab PK bridging ELISA using antibodies HCA286 and HCA287P.

Fig. 1. Golimumab PK bridging ELISA using antibodies HCA286 and HCA287P.

Schematic image of PK bridging ELISA measuring free drug.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.



Fig. 2. Golimumab PK bridging ELISA using antibodies HCA289 and HCA290P.

Fig. 2. Golimumab PK bridging ELISA using antibodies HCA289 and HCA290P.

Schematic image of PK bridging ELISA measuring total drug

Schematic image of PK bridging ELISA measuring total drug. Type 2 anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), drug target (red), type 2 anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.



Fig. 3. Golimumab PK antigen capture ELISA using antibody HCA274.

Fig. 3. Golimumab PK antigen capture ELISA using antibody HCA274.

Schematic image of anti-drug/target complex specific antibody in PK ELISA antigen capture format

Schematic image of PK antigen capture ELISA. Drug target (red), monoclonal antibody drug (gold), drug-target complex detection antibody, monovalent Fab format (purple), labeled with HRP.


ADA Bridging ELISA

Fig. 4. Golimumab ADA bridging ELISA using antibody HCA287.

Fig. 4. Golimumab ADA bridging ELISA using antibody HCA287.

Schematic image of ADA bridging assay.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).



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