Golimumab antibody | AbD25451_hIgG1

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Human anti Golimumab:HRP

Anti-Golimumab Antibody is a recombinant, non-inhibitory, anti-idiotypic antibody in IgG1 format, suitable for bioanalytical assay development for golimumab and biosimilars. It is suitable as the detection antibody in a PK bridging ELISA to measure total drug, paired with HCA289.

Human anti Golimumab

Anti-Golimumab Antibody is a recombinant, non-inhibitory, anti-idiotypic antibody in IgG1 format, suitable for bioanalytical assay development for golimumab and biosimilars. It is suitable as the detection antibody in a PK bridging ELISA to measure total drug, paired with HCA289.

Product Type
Monoclonal Antibody
Clone
AbD25451_hIgG1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
HCA290P
Datasheet
E
SDS
0.1 mg loader

HCA290
Datasheet
E
SDS
0.1 mg loader

Human Anti-Golimumab Antibody, clone AbD25451_hIgG1 is an anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug golimumab. This antibody binds to both free golimumab and to golimumab bound to its target tumor necrosis factor alpha (TNFα). Clone AbD25451_hIgG1 does not inhibit the binding of golimumab to its target TNFα and can be used to measure total golimumab and biosimilar products in bioanalytical assays.

Clone AbD25451_hIgG1 is an affinity-matured variant of AbD20897 (HCA242), with about 17-fold affinity improvement.

A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD25455 (HCA289) as the capture antibody.

Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-golimumab antibodies

Product Details

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Golimumab
Affinity
The monovalent intrinsic affinity of AbD25451_hIgG1 was measured as KD = 0.4 nM by real time, label free molecular interaction analysis on immobilized golimumab
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
Regulatory
For research purposes only

Applications of Golimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA289 (AbD25455, Fab-FH) as the capture reagent.
Protocol: PK bridging ELISA to measure total drug
ELISA
This product may be used in a direct or indirect ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA289 (AbD25455, Fab-FH) as the capture reagent.
Protocol: PK bridging ELISA to measure total drug

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Golimumab HCA289 E 0.1 mg loader
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Golimumab HCA289 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader

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