Golimumab antibody | AbD25451_hIgG1
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25451_hIgG1 (HCA290P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with golimumab, human TNFα, human IgG1/kappa, human IgG1/lambda or adalimumab at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, the human TNFα/golimumab complex was formed by adding 2 μg/ml golimumab to the wells coated with human TNFα. Detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25451_hIgG1 (HCA290P) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with human TNFα at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, golimumab was added (0.3 μg/ml) spiked with increasing concentrations of Human Anti-Golimumab Antibody clone AbD25418 (HCA287) or AbD25451 (HCA290), both in the monovalent Fab format in this assay. Detection of golimumab was performed using Mouse Anti-Human IgG (Fc) CH2 Domain:HRP Antibody (MCA647P) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Golimumab Antibody, clone AbD25455 (HCA289) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of golimumab. Detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25451_hIgG1 (HCA290P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Golimumab Antibody, clone AbD25429 (HCA286) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of golimumab. Detection was performed using Human Anti-Golimumab:HRP Antibody, clone AbD25451_hIgG1 (HCA290P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Golimumab:HRP
Anti-Golimumab Antibody is a recombinant, non-inhibitory, anti-idiotypic antibody in IgG1 format, suitable for bioanalytical assay development for golimumab and biosimilars. It is suitable as the detection antibody in a PK bridging ELISA to measure total drug, paired with HCA289.
Human anti Golimumab
Anti-Golimumab Antibody is a recombinant, non-inhibitory, anti-idiotypic antibody in IgG1 format, suitable for bioanalytical assay development for golimumab and biosimilars. It is suitable as the detection antibody in a PK bridging ELISA to measure total drug, paired with HCA289.
- Product Type
- Monoclonal Antibody
- Clone
- AbD25451_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA290P | E | 0.1 mg |
|
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| HCA290 | E | 0.1 mg |
|
Human Anti-Golimumab Antibody, clone AbD25451_hIgG1 is an anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug golimumab. This antibody binds to both free golimumab and to golimumab bound to its target tumor necrosis factor alpha (TNFα). Clone AbD25451_hIgG1 does not inhibit the binding of golimumab to its target TNFα and can be used to measure total golimumab and biosimilar products in bioanalytical assays.
Clone AbD25451_hIgG1 is an affinity-matured variant of AbD20897 (HCA242), with about 17-fold affinity improvement.
A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD25455 (HCA289) as the capture antibody.
Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
Clone AbD25451_hIgG1 is an affinity-matured variant of AbD20897 (HCA242), with about 17-fold affinity improvement.
A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD25455 (HCA289) as the capture antibody.
Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
Product Details
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Golimumab
- Affinity
- The monovalent intrinsic affinity of AbD25451_hIgG1 was measured as KD = 0.4 nM by real time, label free molecular interaction analysis on immobilized golimumab
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
- Regulatory
- For research purposes only
Applications of Golimumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA289 (AbD25455, Fab-FH) as the capture reagent.
Protocol: PK bridging ELISA to measure total drug - ELISA
- This product may be used in a direct or indirect ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA289 (AbD25455, Fab-FH) as the capture reagent.
Protocol: PK bridging ELISA to measure total drug
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Golimumab | HCA289 | E | 0.1 mg |
|
|
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
|
|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Golimumab | HCA289 | E | 0.1 mg |
|
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| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
| Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
|
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