Antibodies for bioanalysis and drug monitoring of natalizumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug natalizumab (Tysabri) using our range of ready-made anti-idiotypic antibodies.

  • Inhibitory antibodies specific to natalizumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Natalizumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Natalizumab Inhibitory

Type 1

HCA248

AbD21375

Fab-FH1

2.1

PK bridging ELISA

Order HCA248

HCA249

HCA249P

AbD21375_hlgG1

Human IgG1

HRP

2.1

PK bridging ELISA

ADA control

Order HCA249

Order HCA249P

HCA250

AbD21384_hlgG1

Human IgG1

12.2

ADA control

Order HCA250

 * Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Natalizumab Inhibitory Antibodies (Type 1)

Type 1 anti-natalizumab antibodies inhibit the binding of the drug natalizumab to its target, human alpha4-beta1-integrin. They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Natalizumab PK bridging ELISA using antibodies HCA248 and HCA249P.

Fig. 1. Natalizumab PK bridging ELISA using antibodies HCA248 and HCA249P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Natalizumab ADA bridging ELISA using antibody HCA249 or HCA250.

Fig. 2. Natalizumab ADA bridging ELISA using antibody HCA249 or HCA250.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).



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Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.