Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Protocol: PK ELISA Antigen Capture Format For Use With Anti-Golimumab Antibody
Pharmacokinetic (PK) ELISA antigen capture format: For use with anti-golimumab drug/target complex monoclonal antibody product HCA245 or HCA274
This method provides a procedure for carrying out a PK ELISA Antigen Capture Format with anti-golimumab antibodies, product codes HCA245 (IgG1 format) or HCA274 (monovalent Fab format) as detection antibody, and using golimumab monoclonal antibody for the standard curve. HCA274 is an affinity matured antibody that enables development of a higher sensitivity ELISA.
Anti-golimumab drug/target complex antibody recognizes golimumab only when bound to its target TNFα. It does not recognize the free drug or unbound TNFα. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms..
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.