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Protocol: PK ELISA Antigen Capture Format For Use With Anti-Golimumab Antibody
Pharmacokinetic (PK) ELISA antigen capture format: For use with anti-golimumab drug/target complex monoclonal antibody product HCA245 or HCA274
This method provides a procedure for carrying out a PK ELISA Antigen Capture Format with anti-golimumab antibodies, product codes HCA245 (IgG1 format) or HCA274 (monovalent Fab format) as detection antibody, and using golimumab monoclonal antibody for the standard curve. HCA274 is an affinity matured antibody that enables development of a higher sensitivity ELISA.
Anti-golimumab drug/target complex antibody recognizes golimumab only when bound to its target TNFα. It does not recognize the free drug or unbound TNFα. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms..
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