Golimumab antibody | AbD20893_hIgG1
A microtiter plate was coated over night with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti golimumab (clone AbD20893_hIgG1) at a concentration of 2 μg/ml and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtiter plate was coated over night with human TNFα, golimumab, human IgG1/kappa or human IgG1/lambda at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, the TNFα/golimumab complex was formed by adding 2 µg/ml golimumab to TNFα-coated wells. Detection was performed using HRP-conjugated Human anti golimumab (clone AbD20893_hIgG1) titrated to the given concentrations in PBST followed by QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®. Data for the binding of antibody to TNFα/golimumab is presented as the mean of three measurements
A microtiter plate was coated over night with human TNFα at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of golimumab. Detection was performed using HRP-conjugated Human anti golimumab (clone AbD20893_hIgG1) at a concentration of 2 µg/ml in HISPEC assay diluent (BUF049A) followed by QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®. Data for the binding of antibody to TNFα/golimumab is presented as the mean of three measurements
Human anti Golimumab (Drug/Target Complex)
Anti-golimumab drug-target complex antibody specifically recognizes the golimumab-TNF alpha complex and detects golimumab or biosimilars only when bound to TNF alpha. This antibody is in full immunoglobulin format and is suitable for a PK antigen capture format assay to detect bound drug.
- Product Type
- Monoclonal Antibody
- Clone
- AbD20893_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA245 | E | 0.1 mg |
|
Human anti golimumab (drug/target complex) antibody, clone AbD20893_hIgG1 is a Type 3 anti-idiotypic antibody that specifically recognizes golimumab when bound to tumor necrosis factor alpha (TNFα) i.e. the drug/target complex. Clone AbD20893_hIgG1 does not recognize unbound golimumab nor free TNF&alpha. Human anti golimumab (drug/target complex) antibody, clone AbD20893_hIgG1 may be used to measure golimumab when captured by immobilized human TNFα or golimumab/TNFα levels in serum from patients.
Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
Product Details
- Product Form
- Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Golimumab.
- Affinity
- The monovalent intrinsic affinity of AbD20893_hIgG1 was measured as KD = 53 nM by real time, label free molecular interaction analysis on immobilized golimumab in complex with human TNFα.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Golimumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- When conjugated to HRP, this product may be used in a direct ELISA to detect the golimumab/TNFα complex or to detect golimumab bound to immobilized TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK003P | CJ | 1 Conjugation For 4mg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK004P | CJ | 5 Conjugations For 4mg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK005P | CJ | 1 Conjugation For 20mg Antibody | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK006P | CJ | 3 Conjugations For 40µg Antibody |
Product Specific References
References for Golimumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-90.
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