Golimumab antibody | AbD20893_hIgG1

Human anti Golimumab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Clone
AbD20893_hIgG1
Isotype
IgG1
Specificity
Golimumab
Region
DRUG/TARGET COMPLEX

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HCA245
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Human anti golimumab (drug/target complex) antibody, clone AbD20893_hIgG1 is a Type 3 anti-idiotypic antibody that specifically recognizes golimumab when bound to tumor necrosis factor alpha (TNFα) i.e. the drug/target complex. Clone AbD20893_hIgG1 does not recognize unbound golimumab nor free TNF&alpha. Human anti golimumab (drug/target complex) antibody, clone AbD20893_hIgG1 may be used to measure golimumab when captured by immobilized human TNFα or golimumab/TNFα levels in serum from patients.

Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-golimumab antibodies

Product Form
Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid. Simponi is a trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Golimumab/TNFα complex
Affinity
The intrinsic affinity of the monovalent form of Human anti Golimumab antibody, clone AbD20893_hIgG1 is KD = 53 nM as measured by real time, label free molecular interaction analysis on immobilized Golimumab in complex with human TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
When conjugated to HRP, this product may be used in a direct ELISA to detect the golimumab/TNFα complex or to detect golimumab bound to immobilized TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively.

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LYNX Rapid HRP Antibody Conjugation Kit LNK003P CJ 1 Conjugation For 4mg Antibody
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LYNX Rapid HRP Antibody Conjugation Kit LNK004P CJ 5 Conjugations For 4mg Antibody
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LYNX Rapid HRP Antibody Conjugation Kit LNK005P CJ 1 Conjugation For 20mg Antibody
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LYNX Rapid HRP Antibody Conjugation Kit LNK006P CJ 3 Conjugations For 40µg Antibody
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Hispec Assay Diluent BUF049A E IY 50 ml
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References for Golimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-90.

Synonyms
Simponi
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"