Golimumab antibody | AbD20893_hIgG1

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Human anti Golimumab (Drug/Target Complex)

Anti-golimumab drug-target complex antibody specifically recognizes the golimumab-TNF alpha complex and detects golimumab or biosimilars only when bound to TNF alpha. This antibody is in full immunoglobulin format and is suitable for a PK antigen capture format assay to detect bound drug.

Product Type
Monoclonal Antibody
Clone
AbD20893_hIgG1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA245 E 0.1 mg
Human anti golimumab (drug/target complex), clone AbD20893_hIgG1 is a Type 3 anti-idiotypic antibody that specifically recognizes golimumab when bound to tumor necrosis factor alpha (TNFα) i.e. the drug/target complex. Clone AbD20893_hIgG1 does not recognize unbound golimumab nor free TNFα. Clone AbD20893_hIgG1 may be used to measure golimumab when captured by immobilized human TNFα or golimumab/TNFα levels in serum from patients.

Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.

View a summary of all anti-golimumab antibodies

Product Details

Product Form
Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Golimumab.
Affinity
The monovalent intrinsic affinity of AbD20893_hIgG1 was measured as KD = 53 nM by real time, label free molecular interaction analysis on immobilized golimumab in complex with human TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Golimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
When conjugated to HRP, this product may be used in a direct ELISA to detect the golimumab/TNFα complex or to detect golimumab bound to immobilized TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
LYNX Rapid HRP Antibody Conjugation Kit LNK001P 1 Conjugation For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK003P 1 Conjugation For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK004P 5 Conjugations For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK005P 1 Conjugation For 20mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK006P 3 Conjugations For 40µg Antibody CJ