Anti-Abatacept Antibodies

Antibodies for Bioanalysis and Drug Monitoring of Abatacept and Biosimilars

Develop highly selective and sensitive PK and ADA assays for abatacept (Orencia) using our range of ready-made antibodies.

• Recombinant antibodies specific to abatacept CTLA-4 domain
• Fully human format for surrogate positive control or calibrator
• Sequence defined, well characterized reagents with secure supply
• Stringent quality control for batch-to-batch consistency
• Tested for use with mouse anti-human CTLA-4 (CD152) antibody

Table 1. Antibodies Specific to Abatacept and CTLA-4.

* Affinity measured in the monovalent Fab format.
¹ Fab-FH= Monovalent Fab antibody DYKDDDDK- and His-6-tags.
²Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.

Anti-Abatacept Antibodies

Abatacept is a recombinant fusion protein drug consisting of the extracellular domain of human cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), linked to a modified Fc portion of human immunoglobulin G1 (hIgG1). Our anti-abatacept antibodies inhibit the binding of the drug to its target CD80. Human Anti-Abatacept Antibody, clones AbD37058ia (HCA335) and AbD37058pap (TZA0143P), can be used for detection in a pharmacokinetic (PK) bridging ELISA to measure free drug, paired with Mouse Anti-Human CTLA-4 (CD152) Antibody (MCA1724) as capture reagent. HCA335, HCA336, and MCA1724 are suitable as surrogate positive controls or calibrators for an anti-drug antibody (ADA) assay.

ELISA Protocols to Get You Started

PK Bridging ELISA

Fig. 1. Abatacept PK bridging ELISA using antibodies MCA1724 and HCA335.	Schematic image of PK bridging ELISA measuring free drug. Monoclonal capture antibody, Ig format (gray), fusion protein drug (gold-green), anti-drug detection antibody, Ig format (blue) labeled with HRP.

Fig. 2. Abatacept PK bridging ELISA using antibodies MCA1724 (capture) and TZA0143P (detection).	Schematic image of PK bridging ELISA measuring free drug. Monoclonal capture antibody, Ig format (gray), fusion protein drug (gold-green), detection antibody, Fab format (purple) labeled with HRP.

ADA Bridging ELISA

Fig. 3. Abatacept ADA bridging ELISA using antibodies HCA335, HCA336, or MCA1724.	Schematic image of ADA bridging assay. Fusion protein drug as capture and detection reagent labeled with HRP (gold-green), monoclonal anti-drug antibody, Ig format (blue).

Resources

• Anti-idiotypic antibodies, their binding types and properties
• Anti-biotherapeutic antibody quality control and characterization
• Custom anti-idiotypic antibody generation service

Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.