Golimumab antibody | AbD25455
Human anti Golimumab
Anti-Golimumab Antibody is a recombinant, non-inhibitory, anti-idiotypic antibody in monovalent Fab format, suitable for bioanalytical assay development for golimumab and biosimilars. It is suitable as a capture antibody in a PK bridging ELISA to measure total drug, paired with HCA290.
- Product Type
- Monoclonal Antibody
- HuCAL Fab monovalent
|Product Code||Applications||Pack Size||List Price||Quantity|
Clone AbD25455 is an affinity-matured variant of clone AbD20897 (HCA242), with about 7-fold affinity improvement.
A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an antibody in full immunoglobulin format, clone AbD25451_hIgG1 (HCA290), as the detection antibody.
Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- The monovalent intrinsic affinity of AbD25455 was measured as KD = 0.9 nM by real time, label free molecular interaction analysis on immobilized golimumab
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
His-tag is a registered trademark of EMD Biosciences.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- For research purposes only
Applications of Golimumab antibody
|Application Name||Verified||Min Dilution||Max Dilution|
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Clone AbD25455 can be used in a direct or indirect ELISA system or as capture antibody for golimumab in a bridging ELISA together with HCA290 (AbD25455_hIgG1) as the detection reagent.
Protocol: PK bridging ELISA to measure total drug
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Human anti Golimumab||HCA290||E||0.1 mg|
|Human anti Golimumab:HRP||HCA290P||E||0.1 mg|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK001P||CJ||1 Conjugation For 400µg Antibody|
|Mouse anti Penta Histidine Tag:HRP||MCA5995P||E WB||125 µl|
|Mouse anti Human IgG (Fc) CH2 Domain:HRP||MCA647P||C* E||0.2 mg|
Product Specific References
References for Golimumab antibody
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-90.
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