Golimumab antibody | AbD20692_hIgG1
Human anti Golimumab
Anti-golimumab antibody is a recombinant, neutralizing anti-idiotypic fully human IgG1 antibody, suitable for bioanalytical method development for golimumab and biosimilars. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
- Product Type
- Monoclonal Antibody
|Product Code||Applications||Pack Size||List Price||Quantity|
An affinity matured variant of HCA241 is available with about 21-fold affinity improvement, clone AbD25418_hIgG1 (HCA287).
Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- The monovalent intrinsic affinity of AbD20692_hIgG1 was measured as KD = 2.8 nM by real time, label free molecular interaction analysis on immobilized golimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- For research purposes only
Applications of Golimumab antibody
|Application Name||Verified||Min Dilution||Max Dilution|
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Hispec Assay Diluent||BUF049A||E IY||50 ml|
|Human anti Golimumab||HCA240||E||0.1 mg|
|Human anti Golimumab||HCA242||E||0.1 mg|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK001P||CJ||1 Conjugation For 400µg Antibody|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK002P||CJ||3 Conjugations For 400µg Antibody|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK003P||CJ||1 Conjugation For 4mg Antibody|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK004P||CJ||5 Conjugations For 4mg Antibody|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK005P||CJ||1 Conjugation For 20mg Antibody|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK006P||CJ||3 Conjugations For 40µg Antibody|
|Mouse anti Human IgG (Fc) CH2 Domain:HRP||MCA647P||C* E||0.2 mg|
Fluorescent SpectraviewerWatch the Tool Tutorial Video ▸
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra