Antibodies for bioanalysis and drug monitoring of pembrolizumab

Develop highly selective and sensitive PK and ADA assays for the immune checkpoint inhibitor drug pembrolizumab (Keytruda) using our range of ready-made anti-idiotypic antibodies.

  • Inhibitory antibodies specific to pembrolizumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Pembrolizumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Pembrolizumab Inhibitory

Type 1

HCA296

AbD30689

Fab-FH1

0.6

PK bridging ELISA

Order HCA296

HCA297
HCA297P

AbD30685_hIgG1

hIgG1

HRP

5

PK bridging ELISA

ADA control

Order HCA297
Order HCA297P

HCA298

AbD30689_hIgG1

hIgG1

0.6

ADA control

Order HCA298

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Pembrolizumab Inhibitory Antibodies (Type 1)

Type 1 anti-pembrolizumab antibodies inhibit the binding of the drug pembrolizumab to its target, programmed cell death 1 (PD-1). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are available in two affinities, and are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Pembrolizumab PK bridging ELISA using antibodies HCA296 and HCA297P.

Fig. 1. Pembrolizumab PK bridging ELISA using antibodies HCA296 and HCA297P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. ADA bridging ELISA using antibody HCA297.

Fig. 2. Pembrolizumab ADA bridging ELISA using antibody HCA297.

Schematic image of ADA bridging assay.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).


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