Golimumab antibody | AbD20710





Human anti Golimumab
Anti-golimumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development for golimumab and biosimilars. It is suitable as a capture antibody in PK bridging ELISA format for an assay to detect free drug.
- Product Type
- Monoclonal Antibody
- Clone
- AbD20710
- Isotype
- HuCAL Fab monovalent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA240 | E | 0.1 mg |
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An affinity matured variant of HCA240 is available with about 2-fold affinity improvement, clone AbD25429 (HCA286).
Golimumab, marketed under the brand name Simponi, is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
View a summary of all anti-golimumab antibodies
Product Details
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Golimumab.
- Affinity
- The monovalent intrinsic affinity of AbD20710 was measured as KD = 2.3 nM by real time, label free molecular interaction analysis on immobilized golimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Golimumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Golimumab | HCA241 | E | 0.1 mg |
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Human anti Golimumab | HCA242 | E | 0.1 mg |
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LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK003P | CJ | 1 Conjugation For 4mg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK004P | CJ | 5 Conjugations For 4mg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK005P | CJ | 1 Conjugation For 20mg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK006P | CJ | 3 Conjugations For 40µg Antibody |
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