Antibodies for bioanalysis and drug monitoring of tocilizumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for tocilizumab (Actemra, RoActemra) using our range of ready-made anti-idiotypic antibodies.

  • Inhibitory and non-inhibitory antibodies specific to tocilizumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Tocilizumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Tocilizumab

Inhibitory Type 1

HCA252

AbD21362

Fab-FH1

0.9

PK bridging ELISA

Order HCA252

HCA253
HCA253P

AbD21338_hIgG1

hIgG1

HRP

0.1

PK bridging ELISA

ADA control

Order HCA253
Order HCA253P

HCA254

AbD21364_hIgG1

hIgG1

0.3

ADA control

Order HCA254

HCA255

AbD21346_hIgG

hIgG1

6.2

ADA control

Order HCA255

HCA256

AbD21345_hIgG1

hIgG1

15

ADA control

Order HCA256

Tocilizumab & Tocilizumab-IL6R

Non-inhibitory Type 2

HCA257
HCA257P

AbD22155_hIgG1

hIgG1

HRP

31

PK bridging ELISA

 

Order HCA257
Order HCA257P

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Tocilizumab Inhibitory Antibodies (Type 1)

Type 1 anti-tocilizumab antibodies inhibit the binding of the drug tocilizumab to its target, human interleukin-6 receptor (IL-6R). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are available in high, medium and lower affinity, and are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


Anti-Tocilizumab Non-Inhibitory Antibody (Type 2)

The type 2 anti-tocilizumab antibody binds to the tocilizumab idiotope, but does not inhibit the binding of tocilizumab to IL-6R. It can therefore be used in combination with a type 1 antibody in a PK bridging ELISA to measure free and partially bound drug, which is equivalent to total drug when trough serum levels are above 10 µg/ml.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Tocilizumab PK bridging ELISA using antibodies HCA252 and HCA253P.

 

Fig. 1. Tocilizumab PK bridging ELISA using antibodies HCA252 and HCA253P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.



Fig. 2. Tocilizumab PK bridging ELISA using antibodies HCA252 and HCA257P.

 

Fig. 2. Tocilizumab PK bridging ELISA using antibodies HCA252 and HCA257P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free and partially bound drug. Type 1 anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), drug target (red), type 2 anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 3. Tocilizumab ADA bridging ELISA using antibody HCA253, HCA254, HCA255 or HCA256.

 

Fig. 3. Tocilizumab ADA bridging ELISA using antibody HCA253, HCA254, HCA255 or HCA256.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).


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