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Antibodies for bioanalysis and drug monitoring of evolocumab and biosimilars
Develop highly selective and sensitive pharmacokinetic (PK) and anti-drug antibody (ADA) assays for the monoclonal antibody evolocumab (Repatha) using our range of ready-made antibodies.
Table 1. Antibodies Specific to Evolocumab.
Specificity |
Catalog Number |
Clone |
Format |
Affinity* KD, nM |
Assay Recommendation |
Product Details |
---|---|---|---|---|---|---|
Evolocumab Inhibitory Type 1 |
TZA007 |
AbD40406ad |
Fab-F-Spy2-H1 |
1 |
PK bridging ELISA |
|
TZA008 |
AbD40410ad |
Fab-F-Spy2-H1 |
5 |
PK bridging ELISA |
||
HCA376 |
AbD40410ia |
Human IgG1 |
5 |
PK bridging ELISA ADA control |
||
HCA377 |
AbD40406ia |
Human IgG1 |
1 |
ADA control |
||
HCA378 |
AbD40405ia |
Human IgG1 |
5 |
ADA control |
* Affinity measured in the monovalent Fab format.
1 Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
Type 1 anti-evolocumab antibodies inhibit the binding of the monoclonal antibody drug evolocumab to its target, human proprotein convertase subtilisin/kexin type 9 (PCSK9). They are ideal for development of a PK bridging ELISA to measure free drug. Three clones are available in fully human IgG1 format for use as a surrogate positive control or reference standard in an ADA assay.
TrailBlazer Antibodies, clones AbD40406ad (TZA007) and AbD40410ad (TZA008), have a SpyTag2 incorporated at the C-terminus of the antibody heavy chain. In a fast reaction, that simply requires mixing at room temperature, these antibodies can form a covalent isopeptide bond to a SpyCatcher reagent, enabling site-directed conjugation or fast conversion to a bivalent Fab or a full-length Ig-like format within an hour.
Fig. 1. Evolocumab PK bridging ELISA using antibodies TZA007 and HCA376.
Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.
Fig. 2. Evolocumab ADA bridging ELISA using antibody HCA376, HCA377, or HCA378.
Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.