Antibodies for bioanalysis and drug monitoring of vedolizumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug vedolizumab (Entyvio) using ready-made anti-idiotypic antibodies.

  • Inhibitory antibodies specific to vedolizumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Vedolizumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Vedolizumab Inhibitory

Type 1

HCA292

AbD30136

Fab-FH1

0.1

PK bridging ELISA

Order HCA292

HCA293

AbD30136_hIgG1

Human IgG1

0.1

ADA control

Order HCA293

HCA294
HCA294P

AbD30138_hIgG1

Human IgG1

HRP

1.5

PK bridging ELISA

ADA control

Order HCA294
Order HCA294P

HCA295

AbD30139_hIgG1

Human IgG1

4

ADA control

Order HCA295

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Vedolizumab Inhibitory Antibodies (Type 1)

Type 1 anti-vedolizumab antibodies inhibit the binding of the drug vedolizumab to its target, human lymphocyte alpha4-beta7-integrin. They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Vedolizumab PK bridging ELISA using antibodies HCA292 and HCA294P.

Fig. 1. Vedolizumab PK bridging ELISA using antibodies HCA292 and HCA294P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Vedolizumab ADA bridging ELISA using antibody HCA293, HCA294 or HCA295.

Fig. 2. Vedolizumab ADA bridging ELISA using antibody HCA293, HCA294 or HCA295.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).



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Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.