Anti-Pertuzumab Antibodies

s

s

s

Antibodies for bioanalysis and drug monitoring of pertuzumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for pertuzumab (Perjeta) using our range of ready-made antibodies.

• Antibodies specific to pertuzumab or the pertuzumab- HER2 complex
• Fully human format for surrogate positive control or calibrator
• Sequence defined, well characterized reagents with secure supply
• Stringent quality control for batch-to-batch consistency
• Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Pertuzumab and Pertuzumab- HER2 Complex

* Affinity measured in the monovalent Fab format.
¹ Monovalent Fab antibody with (F) DYKDDDDK, and (Sx2) StrepX-StrepX tags.
² Monovalent Fab antibody with (F) DYKDDDDK, and (H) His-6 tags.
³ Fab-F-Spy2-H Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags
⁴ Fab2-FH-X22-HRP Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP conjugated BiCatcher

Anti-Pertuzumab Inhibitory Antibodies (Type 1)

Type 1 anti-pertuzumab antibodies inhibit the binding of the monoclonal antibody drug pertuzumab to its target, human epidermal growth factor receptor 2 (HER2). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. They are available in fully human IgG1 format, in two different affinities, for use as a surrogate positive control or reference standard for an anti-drug antibody (ADA) assay.

Anti-Pertuzumab Non-Inhibitory Antibodies (Type 2)

The type 2 anti-pertuzumab antibodies are specific to the pertuzumab idiotope. They are non-inhibitory anti-idiotypic antibodies that can be used to measure total pertuzumab (free and HER2 bound pertuzumab). 

Anti-Pertuzumab/HER2 Complex Specific Antibodies (Type 3)

The type 3 antibodies specifically recognize the drug-target complex, detecting pertuzumab only when it is bound to HER2. It is suitable as a detection antibody in a PK antigen capture assay.

TrailBlazer Antibodies

TrailBlazer™ Antibodies, clones TZA025 (AbD35569ad) and TZA035 (AbD35572ad) have a SpyTag2 incorporated at the C-terminus of the antibody heavy chain. In a fast reaction that simply requires mixing at room temperature, these antibodies can form a covalent isopeptide bond to a SpyCatcher reagent, enabling site-directed conjugation or fast conversion to a bivalent Fab or a full-length Ig-like format within an hour.

ELISA Protocols to Get You Started

PK Bridging ELISA

Fig. 1. Pertuzumab PK bridging ELISA using antibodies HCA342 and HCA345.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Fig. 2. Pertuzumab PK bridging ELISA using antibodies HCA341 and TZA025P.

Schematic image of PK bridging ELISA measuring free and partially bound drug. Type 1 anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), drug target (red), type 2 anti-idiotypic detection antibody, Fab format (purple), labeled with HRP.

PK Antigen Capture ELISA

Fig. 3. Pertuzumab antigen capture ELISA using antibody TZA035P.

Schematic image of PK antigen capture ELISA. Drug target (red), monoclonal antibody drug (gold), drug target complex detection antibody, monovalent Fab format (purple), labeled with HRP.

ADA Bridging ELISA

Fig. 4. Pertuzumab ADA bridging ELISA using antibody HCA344 or HCA345.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Related Products

Resources

Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.