Antibodies for bioanalysis and drug monitoring of pertuzumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for pertuzumab (Perjeta) using our range of ready-made antibodies.

  • Inhibitory antibodies specific to pertuzumab
  • Fully human format for surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Stringent quality control for batch-to-batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Pertuzumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Pertuzumab

Inhibitory

Type 1

 

HCA341

AbD35668

Fab-FSx21

14

PK bridging ELISA

Order HCA341

HCA342

AbD37070

Fab-FH2

0.7

PK bridging ELISA

Order HCA342

HCA344

AbD35668ia

Human IgG1

14

ADA control

Order HCA344

HCA345

AbD37069ia

Human IgG1

2

PK bridging ELISA

ADA control

Order HCA345

* Affinity measured in the monovalent Fab format.
1 Monovalent Fab antibody with (F) DYKDDDDK, and (Sx2) StrepX-StrepX tags.

2 Monovalent Fab antibody with (F) DYKDDDDK, and (H) His-6 tags.


Anti-Pertuzumab Inhibitory Antibodies (Type 1)

Type 1 anti-pertuzumab antibodies inhibit the binding of the monoclonal antibody drug pertuzumab to its target, human epidermal growth factor receptor 2 (HER2). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. They are available in fully human IgG1 format, in two different affinities, for use as a surrogate positive control or reference standard for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Pertuzumab PK bridging ELISA using antibodies HCA342 and HCA345.

Fig. 1. Pertuzumab PK bridging ELISA using antibodies HCA342 and HCA345.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

 

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Pertuzumab ADA bridging ELISA using antibody HCA344 or HCA345.

Fig. 2. Pertuzumab ADA bridging ELISA using antibody HCA344 or HCA345.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).


Related Products



Resources



Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.