Golimumab antibody | AbD25418_hIgG1
Human anti Golimumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD25418_hIgG1
- Isotype
- IgG1
- Specificity
- Golimumab
Human Anti-Golimumab Antibody, clone AbD25418_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free human monoclonal antibody golimumab and inhibits binding to its target TNFα. Clone AbD25418_hIgG1 does not recognize free human TNFα or golimumab in complex with human TNFα. This antibody can be used to measure free golimumab and biosimilar products in bioanalytical assays. Clone AbD25418_hIgG1 is an affinity-matured variant of AbD20692 (HCA241), with about a 21-fold affinity improvement. A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD25429 (HCA286) in monovalent Fab format as the capture antibody. Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases. View a summary of all anti-golimumab antibodies |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Golimumab
- Affinity
- The monovalent intrinsic affinity of AbD25418 was measured as KD = 0.13 nM by real time, label free molecular interaction analysis on immobilized golimumab
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct or indirect ELISA or as detection antibody for golimumab in a bridging ELISA together with HCA286 (AbD25429) as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
Human anti Golimumab | HCA286 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Golimumab | ||||||
Human anti Golimumab:HRP | HCA287P | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Human anti Golimumab:HRP | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
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Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C * E | 0.2 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Human IgG (Fc) CH2 Domain:HRP |
- Synonyms
- Simponi
HCA287
155815 166181 167939 1703If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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