Antibodies for bioanalysis and drug monitoring of brentuximab vedotin and biosimilars

Develop highly selective and sensitive pharmacokinetic (PK) and anti-drug antibody (ADA) assays for the antibody drug conjugate (ADC) brentuximab vedotin (Adcetris) using our range of ready-made antibodies.

  • Recombinant antibodies specific to brentuximab vedotin
  • Fully human format for surrogate positive control or calibrator
  • Sequence-defined, well characterized reagents with secure supply
  • Stringent quality control for batch-to-batch consistency
  • Nonanimal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Brentuximab Vedotin.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Brentuximab vedotin

Inhibitory

Type 1

HCA349

AbD39649

Fab-FH1

1.3

PK bridging ELISA

Order HCA349

HCA350

AbD39650ia

Human IgG1

6

ADA control

Order HCA350

HCA351

AbD39655ia

Human IgG1

0.7

PK bridging ELISA

ADA control

Order HCA351

HCA352

AbD39659ia

Human IgG1

1.4

ADA control

Order HCA352

* Affinity measured in the monovalent Fab format.
1 Monovalent Fab antibody with (F) DYKDDDDK-, and (H) His-6-tags.


Anti-Brentuximab Vedotin Inhibitory Antibodies (Type 1)

Type 1 anti-brentuximab vedotin antibodies inhibit the binding of brentuximab vedotin to its target, human CD30. They recognize the brentuximab antibody alone, and when it is conjugated to the toxin, monomethyl auristatin E (MMAE). They are ideal for development of a PK bridging ELISA to measure free drug. Three clones are available in fully human IgG1 format for use as a surrogate positive control or reference standard in an ADA assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Brentuximab vedotin PK bridging ELISA using antibodies HCA349 and HCA351.

Fig. 1. Brentuximab vedotin PK bridging ELISA using antibodies HCA349 and HCA351.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Brentuximab vedotin ADA bridging ELISA using antibody HCA350, HCA351, or HCA352.

Fig. 2. Brentuximab vedotin ADA bridging ELISA using antibody HCA350, HCA351, or HCA352.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).


Related Products



Resources



Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.