Antibodies for bioanalysis and drug monitoring of etanercept and biosimilars

Develop highly selective and sensitive PK and ADA assays for etanercept (Enbrel) using our range of ready-made anti-idiotypic antibodies.

  • Inhibitory and non-inhibitory antibodies specific to etanercept p75
  • Antibody recognizing etanercept p75-Fc fusion region
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science 

Table 1. Antibodies Specific to Etanercept.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Etanercept
(p75 TNFR2)
Inhibitory

HCA277
HCA277P

AbD25940_hIgG4Pro

Human

IgG4Pro1

HRP

2.4

PK bridging ELISA
ADA control

Order HCA277
Order HCA277P

Etanercept
(p75 TNFR2)
Non-Inhibitory

HCA276

AbD25939

Fab-FH2

3.2

PK bridging ELISA

 

Order HCA276

Etanercept Fusion-region specific
Non-Inhibitory

HCA279
HCA279P

AbD26183_hIgG1

Human

IgG1

HRP

14.8

PK ELISA

Order HCA279
Order HCA279P

* Affinity measured in the monovalent Fab format
1 IgG4 isotype with a mutation in the core hinge region that prevents the formation of IgG4 half molecules

2 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Etanercept Inhibitory Antibody

Anti-etanercept p75-specific inhibitory antibody

The anti-etanercept inhibitory antibody binds to the p75 subunit of etanercept and blocks the binding of etanercept to its target, tumor necrosis factor alpha (TNFα). It is ideal for use in a bridging ELISA to develop pharmacokinetic (PK) assays to measure free drug, and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay.

Anti-Etanercept Non-Inhibitory Antibody

Anti-etanercept p75-specific non-inhibitory antibody

The anti-etanercept non-inhibitory antibody binds to the p75 subunit of etanercept, but does not inhibit the binding of TNFα. It binds to both free etanercept and to etanercept bound to TNFα and can therefore be used in a bridging ELISA to develop PK assays to detect total drug (free, bound or partially bound).

Anti-Etanercept Fusion-Region Specific Antibody

Anti-etanercept fusion-specific antibody

This antibody specifically recognizes etanercept at the region of the fusion between p75 and the Fc domain of human IgG1. It binds to free etanercept and to etanercept in complex with TNFα. It does not bind to p75 (soluble TNFR2) or to human IgG1. It does not inhibit the binding of etanercept to TNFα. This product may be used as a detection antibody in a bridging ELISA to measure total etanercept very specifically.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Etanercept PK bridging ELISA using antibodies HCA276 and HCA277P.

Fig. 1. Etanercept PK bridging ELISA using antibodies HCA276 and HCA277P.

Schematic image of PK Bridging ELISA. Capture antibody, Fab format (purple), fusion protein drug (gold-green), detection antibody, Ig format (blue), labeled with HRP

Schematic image of PK bridging ELISA measuring free drug. Capture antibody, Fab format (purple), fusion protein drug (gold-green), detection antibody, Ig format (blue), labeled with HRP



Fig. 2. Etanercept PK bridging ELISA using antibodies HCA276 and HCA279P

Fig. 2. Etanercept PK bridging ELISA using antibodies HCA276 and HCA279P

Schematic image of PK bridging ELISA measuring total drug

Schematic image of PK bridging ELISA measuring total drug. Capture antibody, Fab format (purple), fusion protein drug (gold-green), detection antibody, Ig format (blue), labeled with HRP


ADA Bridging ELISA


Fig. 3. Etanercept ADA bridging ELISA using antibody HCA277.

Fig. 3. Etanercept ADA bridging ELISA using antibody HCA277.

Schematic image of ADA bridging assay

Schematic image of ADA bridging assay. Fusion protein drug as capture and detection reagent labeled with HRP (gold-green), fully human anti-drug antibody, Ig format (blue).



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Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.