Antibodies for bioanalysis and drug monitoring of palivizumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug palivizumab (Synagis) using ready-made anti-idiotypic antibodies.

  • Inhibitory antibodies specific to palivizumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Palivizumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Palivizumab Inhibitory

Type 1

HCA261

AbD23967

Fab-FH1

1.5

PK bridging ELISA

Order HCA261

HCA262
HCA262P

AbD23967_hlgG1

Human IgG1

HRP

1.5

PK bridging ELISA

ADA control

Order HCA262
Order HCA262P

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Palivizumab Inhibitory Antibodies (Type 1)

Type 1 anti-palivizumab antibodies inhibit the binding of the drug palivizumab to its target, a highly conserved region on the F protein of respiratory syncytial virus (RSV). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. The antibody in fully human IgG1 format is suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Palivizumab PK bridging ELISA using antibodies HCA261 and HCA262P.

Fig. 1. Palivizumab PK bridging ELISA using antibodies HCA261 and HCA262P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

 

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Palivizumab ADA bridging ELISA using antibody HCA262.

Fig. 2. Palivizumab ADA bridging ELISA using antibody HCA262.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).



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