Protocol: PK Bridging ELISA Detecting Total Drug Using Anti-Golimumab Antibodies
Pharmacokinetic (PK) bridging ELISA protocol, detects total drug (free, partially and fully bound): For use with anti-golimumab monoclonal antibody products HCA240 and HCA243
This method provides a procedure for carrying out a PK ELISA with anti-golimumab antibodies, product codes HCA240 (capture antibody) and HCA243 (detection antibody), and using golimumab monoclonal antibody for the standard curve.
HCA243 anti-golimumab antibody does not inhibit the binding of golimumab to TNFα and can therefore detect total drug – free, partially bound and fully bound. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
Lynx Rapid Conjugation Kit® is a registered trademark of Bio-Rad AbD Serotec Ltd.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.
Payment methods we support: