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PK Bridging ELISA Detecting Total Drug using Anti-Golimumab Antibodies
Pharmacokinetic (PK) bridging ELISA protocol, detects total drug (free, partially and fully bound): for use with Anti-Golimumab Monoclonal Antibodies HCA289 and HCA290P
This method provides a procedure for carrying out a PK ELISA with Anti-Golimumab Antibodies, HCA289 (capture antibody) and HCA290P (detection antibody), and using golimumab for the standard curve. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.