Antibodies for bioanalysis and drug monitoring of adalimumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for adalimumab (Humira) using our range of ready-made anti-idiotypic antibodies.

  • Highly specific to adalimumab or the adalimumab-TNFα complex
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Adalimumab and Adalimumab-TNFα Complex.

Specificity

Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Adalimumab

Inhibitory

Type 1

HCA202

AbD18654

Fab-FH1

0.15

PK bridging ELISA

Order HCA202

HCA203

AbD18654_hIgG1

hIgG1

0.15

ADA control

Order HCA203

HCA204
HCA204P

AbD18655_hIgG1

hIgG1

HRP

0.06

PK bridging ELISA

ADA control

Order HCA204
Order HCA204P

HCA271

AbD18655_hIgE

hIgE

0.06

ADA control
IgE isotype specific

Order HCA271

HCA205

AbD18664_hIgG1

hIgG1

10.5

ADA control

Order HCA205

Adalimumab-TNFα Complex Specific

Type 3

HCA206

AbD18754

Fab-FH1

67

PK ELISA antigen capture format

Order HCA206

HCA207

AbD18754_hIgG1

hIgG1

67

Order HCA207

HCA2312

AbD20350

Fab-FH1

3.2

Order HCA2312

HCA2322
HCA232P

AbD20349_hIgG1

hIgG1

HRP

6.7

Order HCA2322
Order HCA232P

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags

2 HCA231 and HCA232/HCA232P are affinity matured versions of clone AbD18754 (HCA206 and HCA207, respectively)


Anti-Adalimumab Inhibitory Antibodies (Type 1)

Type 1 anti-adalimumab antibodies inhibit the binding of the drug adalimumab to its target, tumor necrosis factor alpha, TNFα. They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


Anti-Adalimumab-TNFα Complex-Specific Antibody (Type 3)

The Type 3 antibody specifically recognizes the drug-target complex, detecting adalimumab only when it is bound to TNFα. It is suitable as a detection antibody in a PK antigen capture assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Adalimumab PK bridging ELISA using antibodies HCA202 and HCA204P.

Fig. 1. Adalimumab PK bridging ELISA using antibodies HCA202 and HCA204P.

Schematic image of PK bridging ELISA. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


PK Antigen Capture ELISA


Fig. 2. Adalimumab PK antigen capture ELISA using antibody HCA231.

Fig. 2. Adalimumab PK antigen capture ELISA using antibody HCA231.

Schematic image of PK antigen capture ELISA. Drug target (red), monoclonal antibody drug (gold), drug target complex detection antibody, Fab format (purple), labeled with HRP.

Schematic image of PK antigen capture ELISA. Drug target (red), monoclonal antibody drug (gold), drug target complex detection antibody, monovalent Fab format (purple), labeled with HRP.


Anti-Drug Antibody Bridging ELISA


Fig. 3. Adalimumab ADA bridging ELISA using antibodies HCA203, HCA204 and HCA205.

Fig. 3. Adalimumab ADA bridging ELISA using antibodies HCA203, HCA204 and HCA205.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).


IgE Isotype Specific ADA Bridging ELISA


Fig. 4. Adalimumab IgE isotype ADA bridging ELISA using antibody HCA271.

Fig. 4. Adalimumab IgE isotype ADA bridging ELISA using antibody HCA271.

Schematic image of ADA assay for IgE isotype detection. Monoclonal antibody drug as capture antibody (gold), fully human anti-idiotypic antibody, IgE format (blue), detection with HRP conjugated anti-human IgE antibody (gray).

Schematic image of ADA assay for IgE isotype detection. Monoclonal antibody drug as capture antibody (gold), fully human anti-idiotypic antibody, IgE format (blue), detection with HRP conjugated anti-human IgE antibody (gray).


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