Antibodies for bioanalysis and drug monitoring of panitumumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug panitumumab (Vectibix) using ready-made anti-idiotypic antibodies.

  • Inhibitory antibodies specific to panitumumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Panitumumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Panitumumab Inhibitory

Type 1

HCA264

AbD23894

Fab-FH1

0.3

PK bridging ELISA

Order HCA264

HCA265
HCA265P

AbD23897_hIgG1

Human IgG1

HRP

0.3

PK bridging ELISA

ADA control

Order HCA265
Order HCA265P

HCA266

AbD23895_hIgG1

Human IgG1

0.6

ADA control

Order HCA266

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Panitumumab Inhibitory Antibodies (Type 1)

Type 1 anti-panitumumab antibodies inhibit the binding of the drug panitumumab to its target, epidermal growth factor receptor (EGFR). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Panitumumab PK bridging ELISA using antibodies HCA264 and HCA265P.

Fig. 1. Panitumumab PK bridging ELISA using antibodies HCA264 and HCA265P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Panitumumab ADA bridging ELISA using antibody HCA265 or HCA266.

Fig. 2. Panitumumab ADA bridging ELISA using antibody HCA265 or HCA266.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).



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