Antibodies for bioanalysis and drug monitoring of eculizumab and biosimilars

Develop highly selective and sensitive PK and ADA assays for the monoclonal antibody drug eculizumab (Soliris) using ready-made anti-idiotypic antibodies.

  • Inhibitory antibodies specific to eculizumab
  • Fully human surrogate positive control or calibrator
  • Sequence defined, well characterized reagents with secure supply
  • Recombinant production, stringent quality control for batch to batch consistency
  • Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Eculizumab.

Specificity
Binding Type

Catalog Number

Clone

Format

Affinity* KD, nM

Assay Recommendation

Product Details

Eculizumab Inhibitory

Type 1

HCA312

AbD32311

Fab-FH1

6

PK bridging ELISA

Order HCA312

HCA313
HCA313P

AbD32107_hIgG1

Human IgG1

HRP

0.6

PK bridging ELISA

ADA control

Order HCA313
Order HCA313P

HCA314

AbD32311_hIgG1

Human IgG1

6

ADA control

Order HCA314

HCA315

AbD32109_hIgG1

Human IgG1

3

ADA control

Order HCA315

* Affinity measured in the monovalent Fab format
1 Monovalent Fab antibody DYKDDDDK- and His-6-tags


Anti-Eculizumab Inhibitory Antibodies (Type 1)

Type 1 anti-eculizumab antibodies inhibit the binding of the drug eculizumab to its target, human complement C5 protein (C5). They are ideal for development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In fully human IgG1 format they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.


ELISA Protocols to Get You Started

PK Bridging ELISA


Fig. 1. Eculizumab PK bridging ELISA using antibodies HCA312 and HCA313P.

Fig. 1. Eculizumab PK bridging ELISA using antibodies HCA312 and HCA313P.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.

Schematic image of PK bridging ELISA measuring free drug. Anti-idiotypic capture antibody, Fab format (purple), monoclonal antibody drug (gold), anti-idiotypic detection antibody, Ig format (blue), labeled with HRP.


ADA Bridging ELISA


Fig. 2. Eculizumab ADA bridging ELISA using antibody HCA313, HCA314 or HCA315.

Fig. 2. Eculizumab ADA bridging ELISA using antibody HCA313, HCA314 or HCA315.

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).

Schematic image of ADA bridging assay. Monoclonal antibody drug as capture antibody and detection antibody labeled with HRP (gold), fully human anti-idiotypic antibody, Ig format (blue).



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Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.