Adalimumab antibody | AbD18754

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Human anti Adalimumab (Drug/Target Complex)

Anti-adalimumab drug-target complex antibody specifically recognizes the adalimumab-TNF alpha complex exclusively and detects adalimumab or biosimilars only when bound to TNF alpha. This antibody is in monovalent Fab format and is suitable for a PK antigen capture format assay.

Product Type
Monoclonal Antibody
Clone
AbD18754
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
HCA206
Datasheet
E
SDS
0.1 mg loader

Human anti Adalimumab, clone AbD18754, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα.

Clone AbD18754 can be used in assays to measure adalimumab that has been captured via immobilized TNFα. It can also be used to measure adalimumab, TNFα and biosimilar products using bioanalytical assays.

Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all anti-adalimumab antibodies

Product Details

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Adalimumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=67 nM by real time, label-free molecular interaction analysis on immobilized adalimumab/TNFα complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira® is a registered trademark of Abbott Laboratories.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Adalimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA on Adalimumab/TNFα complexes or as detection antibody, when conjugated to HRP, for Adalimumab bound to TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti DYKDDDDK Tag:HRP AHP1074P E WB 0.1 mg loader
Goat anti Histidine Tag:Biotin AHP1656B E WB 0.1 mg loader
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg loader
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

Product Specific References

References for Adalimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-190.

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