Adalimumab antibody | AbD18754



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E ResetHuman anti Adalimumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD18754
- Isotype
- HuCAL Fab monovalent
- Specificity
- Adalimumab
- Region
- (DRUG/TARGET COMPLEX)
Human anti Adalimumab, clone AbD18754, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα. Clone AbD18754 can be used in assays to measure adalimumab that has been captured via immobilized TNFα. It can also be used to measure adalimumab, TNFα and biosimilar products using bioanalytical assays. Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases. View a summary of all anti-adalimumab antibodies |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Adalimumab/TNFα complex
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=67 nM as measured by real time, label-free molecular interaction analysis on immobilized Adalimumab/TNFα complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira® is a registered trademark of Abbott Laboratories.
His-tag is a registered trademark of EMD Biosciences.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
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ELISA | ![]() |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA on Adalimumab/TNFα complexes or as detection antibody, when conjugated to HRP, for Adalimumab bound to TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
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Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg |
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Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Description | Mouse anti Histidine Tag:Alk.Phos. | ||||||
Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Description | Mouse anti Histidine Tag:Biotin | ||||||
Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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Description | Rat anti DYKDDDDK Tag:HRP | ||||||
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Description | Mouse anti Penta Histidine Tag:HRP |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Hispec Assay Diluent | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
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Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
Recombinant Human TNF Alpha | PHP051 | E FN WB | 50 µg | Log in | |||
List Price | Your Price | ||||||
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Description | Recombinant Human TNF Alpha | ||||||
Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml | Log in | |||
List Price | Your Price | ||||||
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Description | Goat anti Human IgG F(ab')2:HRP |
References for Adalimumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
- Synonyms
- HUMIRA®
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA206

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