Adalimumab antibody | AbD18754
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Adalimumab Antibody, clone AbD18754 (HCA206) at a concentration of 2 μg/ml followed by Mouse Anti-Strep-Tag Classic:HRP Antibody (MCA2489P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Recombinant Human TNFα (PHP051) at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of adalimumab. Detection was performed using HRP conjugated Human Anti-Adalimumab Antibody, clone AbD18754 (HCA206) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with Recombinant Human TNFα (PHP051), adalimumab, infliximab, Human IgG1 kappa control and BSA at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, the Human TNFα/adalimumab and TNFα/infliximab complexes were formed by adding 2 μg/ml adalimumab or infliximab to the wells coated with TNFα. Detection was performed using Human Anti-Adalimumab Antibody, clone AbD18754 (HCA206) titrated to the given concentrations in PBST followed by Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Filter by Application:
E ResetHuman anti Adalimumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD18754
- Isotype
- HuCAL Fab monovalent
- Specificity
- Adalimumab
- Region
- (DRUG/TARGET COMPLEX)
Antibody Quality and Performance Guarantee
| Human anti Adalimumab, clone AbD18754, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα. Clone AbD18754 can be used in assays to measure adalimumab that has been captured via immobilized TNFα. It can also be used to measure adalimumab, TNFα and biosimilar products using bioanalytical assays. Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases. View a summary of all anti-adalimumab antibodies |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Adalimumab/TNFα complex
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=67 nM as measured by real time, label-free molecular interaction analysis on immobilized Adalimumab/TNFα complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira® is a registered trademark of Abbott Laboratories.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA on Adalimumab/TNFα complexes or as detection antibody, when conjugated to HRP, for Adalimumab bound to TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg |
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| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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| Description | Rat anti DYKDDDDK Tag:HRP | ||||||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
| Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg |
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| Description | Rabbit anti DYKDDDDK Tag:HRP | ||||||
| Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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| Description | Mouse anti Histidine Tag:Alk.Phos. | ||||||
| Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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| Description | Mouse anti Histidine Tag:Biotin | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml |
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Recombinant Human TNF Alpha | PHP051 | E FN WB | 50 µg |
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| List Price | Your Price | ||||||
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| Description | Recombinant Human TNF Alpha | ||||||
References for Adalimumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
- Synonyms
- HUMIRA®
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA206
0912
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