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PK ELISA Antigen Capture Format For Use With Anti-Adalimumab Antibody
Pharmacokinetic (PK) ELISA Antigen Capture Format: For use with anti-adalimumab monoclonal antibody products HCA231 or HCA232/HCA232P
This method provides a procedure for carrying out a PK ELISA Antigen Capture Format with anti-adalimumab antibodies, product codes HCA231 or HCA232P (detection antibody), and using adalimumab monoclonal antibody for the standard curve.
Anti-adalimumab drug/target complex antibody recognizes adalimumab only when bound to its target TNFα. It does not recognize the free drug or unbound TNFα. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
Lynx Rapid Conjugation Kit® is a registered trademark of Bio-Rad AbD Serotec Ltd.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.