Adalimumab antibody | AbD18754_hIgG1

Human anti Adalimumab (Drug/Target Complex)

Product Type
Monoclonal Antibody
Clone
AbD18754_hIgG1
Isotype
IgG1
Specificity
Adalimumab
Region
(DRUG/TARGET COMPLEX)

Product Code Applications Pack Size List Price Your Price Qty
HCA207
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Human anti Adalimumab, clone AbD18754_hIgG1, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754_hIgG1 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα.

Clone AbD18754_hIgG1 can be used in assays to measure adalimumab that has been captured via immobilized TNFα. It can also be used to measure adalimumab/TNFα and biosimilar products using bioanalytical assays.

Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all anti-adalimumab antibodies

Product Form
Human IgG1 antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Adalimumab/TNFα complex
Affinity
The intrinsic affinity of the monovalent form of this antibody is KD=67 nM as measured by real time, label-free molecular interaction analysis on immobilized Adalimumab/TNFα.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira® is a registered trademark of Abbott Laboratories.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA on adalimumab/TNFα complexes or as detection antibody, when conjugated to HRP, for adalimumab bound to TNFα.
Protocol: PK antigen capture ELISA to measure bound drug exclusively

Description Product Code Applications Pack Size List Price Your Price Quantity
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
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loader
Description Recombinant Human TNF Alpha
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent

References for Adalimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-190.

Synonyms
HUMIRA®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Adalimumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"