TNF Alpha antibody | D2E7

Human anti TNF Alpha (Adalimumab Biosimilar)

Product Type
Monoclonal Antibody
Clone
D2E7
Isotype
IgG1
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA6141
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Human Anti-TNF alpha antibody, clone D2E7 is a research grade biosimilar of the monoclonal antibody drug adalimumab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic adalimumab antibody clone D2E7. This product is supplied in a research grade format, i.e. in PBS buffer with preservative, it is not manufactured or formulated in the same way as the therapeutic reference product. A preservative free version is available on request.

Human Anti-TNF alpha antibody, clone D2E7 is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-adalimumab antibodies, for example HCA202 and HCA203. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-adalimumab antibody in IgG1 format as reference standard, for example HCA203, HCA204 or HCA205, or an anti-adalimumab antibody in IgE format as reference standard, for example HCA271.

The therapeutic antibody adalimumab (Humira) is a fully human monoclonal antibody (IgG1 kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNF alpha, and acts by blocking the activation of TNF receptors by TNF alpha. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

This product is NOT FOR THERAPEUTIC USE.

View a summary of supporting anti-adalimumab antibody products

Target Species
Human
Product Form
Human IgG1 (kappa) antibody - liquid
Preparation
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Human TNFα
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Adalimumab HCA202 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab
Human anti Adalimumab HCA203 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab
Human anti Adalimumab HCA204 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab
Human anti Adalimumab:HRP HCA204P E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab:HRP
Human anti Adalimumab HCA205 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab
Human anti Adalimumab (Drug/Target Complex) HCA206 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab (Drug/Target Complex)
Human anti Adalimumab (Drug/Target Complex) HCA207 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab (Drug/Target Complex)
Human anti Adalimumab (Drug/Target Complex) HCA231 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab (Drug/Target Complex)
Human anti Adalimumab (Drug/Target Complex) HCA232 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab (Drug/Target Complex)
Human anti Adalimumab (Drug/Target Complex):HRP HCA232P E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab (Drug/Target Complex):HRP
Human anti Adalimumab HCA271 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Adalimumab
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit

Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
View more products with TNF ALPHA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products