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Protocol: PK Bridging ELISA For Use With Anti-Adalimumab Antibodies
Pharmacokinetic (PK) Bridging ELISA: For use with anti-adalimumab monoclonal antibody products HCA202 and HCA204P
This method provides a procedure for carrying out a PK ELISA with anti-adalimumab antibodies, product codes HCA202 (capture antibody) and HCA204P (detection antibody), and using adalimumab monoclonal antibody for the standard curve.
The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
Lynx Rapid Conjugation Kit® is a registered trademark of Bio-Rad AbD Serotec Ltd.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.