Adalimumab antibody | AbD18655_hIgE
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti adalimumab (clone AbD18655_hIgE) at a concentration of 2 μg/ml followed by Mouse anti human IgE conjugated to HRP in HISPEC diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with adalimumab, human IgG1/kappa or human IgG1/lambda control at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti Adalimumab (clone AbD18655_hIgE) titrated to the given concentrations in PBST+10% NHS, followed by Mouse anti human IgE conjugated to HRP in HISPEC diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data is presented as the mean of three measurements.
Human anti Adalimumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD18655_hIgE
- Isotype
- IgE
- Specificity
- Adalimumab
Antibody Quality and Performance Guarantee
| Human anti Adalimumab, clone AbD18655_hIgE, is an ultra-high affinity, recombinant anti-idiotypic antibody that specifically recognizes adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. This antibody is derived from the same parent clone as HCA204 and has been converted to an IgE isotype. Repeated administration of biotherapeutic drugs can result in the induction of anti-drug antibodies (ADAs), which may affect efficacy of the drug and may cause adverse events including hypersensitivity reactions. Most ADAs have isotype IgG, but ADAs with isotype IgE can potentially elicit anaphylactic reactions. The FDA draft guidance for immunogenicity testing of therapeutic proteins indicates that where the risk of anaphylaxis is a concern, assays should be developed to investigate ADAs with IgE isotype. Clone AbD18655_hIgE is a fully human antibody with isotype IgE, ideal for use as a reference standard in an ELISA to measure IgE ADAs in patient serum. Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases. View a summary of all anti-adalimumab antibodies. |
|
- Product Form
- Human IgE antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgE prepared by affinity chromatography
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Adalimumab (Humira®)
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=0.06 nM as measured by real time, label-free molecular interaction analysis on immobilized adalimumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira is a trademark of Abbott Laboratories. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in an indirect ELISA.
This antibody is fully human and can be used as a reference standard in an ADA assay.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human IgE:HRP | MCA2116P | E | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Human IgE:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049B | E IY | 125 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
Further Reading
-
Wu, Y., et al. (2013) An electrochemiluminescence (ECL)-based assay for the specific detection of anti-drug antibodies of the IgE isotype.
J Pharm Biomed Anal. 86: 73-81. -
Puxeddu, I., et al. (2016) Hypersensitivity reactions during treatment with biological agents.
Clin Exp Rheumatol.34: 129-32.
- Synonyms
- HUMIRA®
HCA271
1610
168418
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with ADALIMUMAB specificity
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