Adalimumab antibody | AbD18655_hIgG1

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Human anti Adalimumab:HRP

Anti-adalimumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human IgG1 format; it is ideal for bioanalytical assay development to measure levels of adalimumab or biosimilars. It is recommended as a detection antibody in a PK bridging ELISA with HCA202.

Human anti Adalimumab

Anti-adalimumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human IgG1 format; it is ideal for bioanalytical assay development to measure levels of adalimumab or biosimilars. It is recommended as a detection antibody in a PK bridging ELISA with HCA202.

Product Type
Monoclonal Antibody
Clone
AbD18655_hIgG1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA204P E 0.1 mg
HCA204 E 0.1 mg
HCA204G E 1 mg
Human Anti-Adalimumab, clone AbD18655_hIgG1, is an ultra-high affinity anti-idiotypic antibody that specifically recognizes adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. Clone AbD18655_hIgG1 can be used to measure the levels of adalimumab and biosimilar products using bioanalytical assays. It may also be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient serum.

Adalimumab (branded as Humira) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all Anti-Adalimumab Antibodies.

Product Details

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Adalimumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.06 nM by real time, label-free molecular interaction analysis on immobilized adalimumab
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Humira® is a registered trademark of Abbott Laboratories.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Humira is a registered trademark of Abbott Laboratories.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Adalimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with HCA202 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
ELISA
This product may be used in an indirect ELISA or, when conjugated to HRP, in a bridging ELISA together with HCA202 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay.
Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Adalimumab HCA202 0.1 mg E
Human anti Adalimumab HCA203 0.1 mg E
Human anti Adalimumab HCA205 0.1 mg E
Human anti Adalimumab (Drug/Target Complex) HCA207 0.1 mg E
Recombinant Human TNF Alpha PHP051 50 µg E FN WB
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Adalimumab HCA202 0.1 mg E
Human anti Adalimumab HCA203 0.1 mg E
Human anti Adalimumab HCA205 0.1 mg E
Human anti Adalimumab (Drug/Target Complex) HCA207 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P 0.2 mg C* E
Recombinant Human TNF Alpha PHP051 50 µg E FN WB

Product Specific References

References for Adalimumab antibody

  1. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    Patent Application US20170315118A1