Adalimumab antibody | AbD20349_hIgG1
A microtiter plate was coated over night with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti adalimumab, clone AbD20349_hIgG1 (HCA232P) at a concentration of 2 μg/ml, followed by QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated over night with various antigens. For TNFα/drug complex antigens, human TNFα was immobilized to the plate. After washing and blocking with PBST+5% BSA, adalimumab or infliximab was added. After further incubation and washing steps, detection was performed using HRP-conjugated Human anti adalimumab, clone AbD20349_hIgG1 (HCA232P) titrated to the given concentrations in PBST, followed by QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with Recombinant Human TNFα (PHP051) at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of adalimumab. Detection was performed using HRP conjugated Human Anti-Adalimumab Antibody, clone AbD20349_hIgG1 (HCA232P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Adalimumab (Drug/Target Complex):HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD20349_hIgG1
- Isotype
- IgG1
- Specificity
- Adalimumab
- Region
- (DRUG/TARGET COMPLEX)
Antibody Quality and Performance Guarantee
| Human anti Adalimumab (drug/target complex), clone AbD20349_hIgG1, is an affinity matured, Type 3 antibody that specifically recognizes adalimumab complexed with TNFα. It does not recognize the free drug or unbound TNFα. Clone AbD20349_hIgG1 can be used in assays to measure adalimumab that has been captured via immobilized TNFα, thereby avoiding the bridging assay format. It can also be used to measure the level of the drug/target complex and biosimilar products using bioanalytical assays. Adalimumab (brand name Humira®) is a human monoclonal antibody (IgG1/kappa) approved for the treatment of rheumatoid, psoriatic and juvenile idiopathic arthritis, ankylosing spondylitis, Crohn’s disease and ulcerative colitis. This therapeutic antibody is directed against the pro-inflammatory cytokine tumor necrosis factor alpha (TNFα), blocking its interaction with the p55 (TNFR1, CD120a) and p75 (TNFR2, CD120b) cell surface TNF receptors, resulting in a down-regulation of the abnormal inflammatory response that is characteristic of autoimmune disease. View a summary of all anti-adalimumab antibodies. |
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Adalimumab/TNFα complex
- Affinity
- The monovalent intrinsic affinity of the monovalent form of this antibody in an unconjugated format is KD=6.7 nM as measured by real time, label-free molecular interaction analysis on the immobilized adalimumab/TNFα complex.
- Approx. Protein Concentrations
- Total protein concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira is a trademark of Abbott Laboratories.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product is suitable for use in direct ELISA applications.
Protocol: PK antigen capture ELISA to measure bound drug exclusively.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Hispec Assay Diluent | ||||||
| Recombinant Human TNF Alpha | PHP051 | E FN WB | 50 µg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Recombinant Human TNF Alpha | ||||||
References for Adalimumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1 -
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
- Synonyms
- HUMIRA®
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA232P
155001
INN1709
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