Adalimumab antibody | AbD18654
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Adalimumab Antibody, clone AbD18654 (HCA202) was added at a concentration of 2 μg/ml. Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Human Anti-Adalimumab Antibody, AbD18654 (HCA202) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of adalimumab. Detection was performed using Human Anti-Adalimumab:HRP Antibody, clone AbD18655_hIgG1 (HCA204P) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with adalimumab (black), infliximab (red), alemtuzumab (green), human IgG1/kappa (blue), BSA (light blue) at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Adalimumab Antibody, clone AbD18654 (HCA202) was added . Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Recombinant Human TNFα (PHP051) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of adalimumab (0.3 μg/ml), spiked with increasing concentrations of Human Anti-Adalimumab Antibody, clone AbD18654 (HCA202) was added. Free adalimumab still capable of binding to the Human Recombinant TNFα coated plate, was detected using Goat Anti-Human IgG F(ab’)2:HRP (STAR126P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Filter by Application:
E ResetHuman anti Adalimumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD18654
- Isotype
- HuCAL Fab monovalent
- Specificity
- Adalimumab
Antibody Quality and 
Unrivaled 
| Human Anti-Adalimumab Antibody, clone AbD18654, is a high affinity anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug adalimumab and inhibits binding to its target, TNFα. The antibody can be used to measure the levels of adalimumab and biosimilar products in bioanalytical assays. Clone AbD18654 is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay when used in combination with HRP-conjugated detection antibody (HCA204P). The assay will measure free drug, as both antibodies are inhibitory. Adalimumab (branded as Humira) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases. View a summary of all Anti-Adalimumab Antibodies. |
- Product Form
- Pack Size: 1 mgA monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.Pack Size: 0.1 mgA monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Adalimumab
- Affinity
- The intrinsic affinity of the monovalent form of of this antibody is KD=0.15 nM as measured by real time, label-free molecular interaction analysis on immobilized adalimumab.
- Approx. Protein Concentrations
- Pack Size: 1 mgAntibody concentration 1.0 mg/mlPack Size: 0.1 mgAntibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira is a trademark of Abbott Laboratories.
His-tag is a trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in an indirect ELISA or in an ELISA bridging assay as a capture antibody together with HCA204P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug.
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References for Adalimumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
- Synonyms
- Humira
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Adalimumab specificity
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