Adalimumab antibody | AbD18654
Human anti Adalimumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD18654
- Isotype
- HuCAL Fab monovalent
- Specificity
- Adalimumab
Human Anti-Adalimumab Antibody, clone AbD18654, is a high affinity anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug adalimumab and inhibits binding to its target, TNFα. The antibody can be used to measure the levels of adalimumab and biosimilar products in bioanalytical assays. Clone AbD18654 is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay when used in combination with HRP-conjugated detection antibody (HCA204P). The assay will measure free drug, as both antibodies are inhibitory. Adalimumab (branded as Humira) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases. View a summary of all Anti-Adalimumab Antibodies. |
- Product Form
-
HCA202G
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid. -
HCA202
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid. - Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Adalimumab
- Affinity
- HCA202G: The intrinsic affinity of the monovalent form of this antibody is KD=0.15 nM as measured by real time, label-free molecular interaction analysis on immobilized adalimumab.
- HCA202: The intrinsic affinity of the monovalent form of of this antibody is KD=0.15 nM as measured by real time, label-free molecular interaction analysis on immobilized adalimumab.
- Approx. Protein Concentrations
- HCA202G: Antibody concentration 1.0 mg/ml
- HCA202: Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira is a trademark of Abbott Laboratories.
His-tag is a trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in an indirect ELISA or in an ELISA bridging assay as a capture antibody together with HCA204P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rabbit anti DYKDDDDK Tag:HRP | AHP1074P | E WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Rabbit anti DYKDDDDK Tag:HRP | ||||||
Goat anti Histidine Tag:Biotin | AHP1656B | E WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Goat anti Histidine Tag:Biotin | ||||||
Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse anti Histidine Tag:Alk.Phos. | ||||||
Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Histidine Tag:Biotin | ||||||
Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Rat anti DYKDDDDK Tag:HRP | ||||||
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Penta Histidine Tag:HRP |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Hispec Assay Diluent | ||||||
Human anti Adalimumab | HCA204 | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Adalimumab | ||||||
Human anti Adalimumab:HRP | HCA204P | E | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human anti Adalimumab:HRP | ||||||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C * E | 0.2 mg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse anti Human IgG (Fc) CH2 Domain:HRP | ||||||
Recombinant Human TNF Alpha | PHP051 | E FN WB | 50 µg | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Recombinant Human TNF Alpha | ||||||
Goat anti Human IgG F(ab')2:HRP | STAR126P | E WB | 0.5 ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Goat anti Human IgG F(ab')2:HRP |
References for Adalimumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
- Synonyms
- Humira
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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