Adalimumab antibody | AbD18654

Human anti Adalimumab

Product Type
Monoclonal Antibody
Clone
AbD18654
Isotype
HuCAL Fab monovalent
Specificity
Adalimumab

Product Code Applications Pack Size List Price Your Price Qty
HCA202G
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SDS Safety Datasheet SDS
E 1 mg loader
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loader
HCA202
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
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Human Anti-Adalimumab Antibody, clone AbD18654, is a high affinity anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug adalimumab and inhibits binding to its target, TNFα. The antibody can be used to measure the levels of adalimumab and biosimilar products in bioanalytical assays.

Clone AbD18654 is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay when used in combination with HRP-conjugated detection antibody (HCA204P). The assay will measure free drug, as both antibodies are inhibitory.

Adalimumab (branded as Humira) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all Anti-Adalimumab Antibodies.

Product Form
Pack Size: 1 mg
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Pack Size: 0.1 mg
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL? phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Adalimumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.15 nM by real time, label-free molecular interaction analysis on immobilized adalimumab.
Approx. Protein Concentrations
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira is a registered trademark of Abbott Laboratories.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or in an ELISA bridging assay as a capture antibody together with HCA204P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti DYKDDDDK Tag:HRP AHP1074P E WB 0.1 mg loader
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Description Rabbit anti DYKDDDDK Tag:HRP
Goat anti Histidine Tag:Biotin AHP1656B E WB 0.1 mg loader
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Description Goat anti Histidine Tag:Biotin
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg loader
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Description Mouse anti Histidine Tag:Alk.Phos.
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
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Description Mouse anti Histidine Tag:Biotin
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg loader
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Description Rat anti DYKDDDDK Tag:HRP
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
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Description Mouse anti Penta Histidine Tag:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Adalimumab HCA204 E 0.1 mg loader
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loader
Description Human anti Adalimumab
Human anti Adalimumab:HRP HCA204P E 0.1 mg loader
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loader
Description Human anti Adalimumab:HRP
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
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loader
Description Mouse anti Human IgG (Fc) CH2 Domain:HRP
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
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loader
Description Recombinant Human TNF Alpha
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader
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Description Goat anti Human IgG F(ab')2:HRP

References for Adalimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-190.

Synonyms
Humira
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"