Adalimumab antibody | AbD18654

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Human anti Adalimumab

Anti-adalimumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development to measure levels of adalimumab or biosimilars. It is recommended as capture antibody in PK bridging ELISA with HCA204/HCA204P.

Product Type
Monoclonal Antibody
Clone
AbD18654
Isotype
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
HCA202
Datasheet
E
MSDS
0.1 mg loader

HCA202G
Datasheet
E
MSDS
1 mg loader

Human Anti-Adalimumab Antibody, clone AbD18654, is a high affinity anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug adalimumab and inhibits binding to its target, TNFα. The antibody can be used to measure the levels of adalimumab and biosimilar products in bioanalytical assays.

Clone AbD18654 is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay when used in combination with HRP-conjugated detection antibody (HCA204P). The assay will measure free drug, as both antibodies are inhibitory.

Adalimumab (branded as Humira) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all Anti-Adalimumab Antibodies.

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Adalimumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.15 nM by real time, label-free molecular interaction analysis on immobilized adalimumab.
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Humira is a registered trademark of Abbott Laboratories.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Adalimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or in an ELISA bridging assay as a capture antibody together with HCA204P as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti DYKDDDDK Tag:HRP AHP1074P E WB 0.1 mg loader
Goat anti Histidine Tag:Biotin AHP1656B E WB 0.1 mg loader
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
Rat anti DYKDDDDK Tag:HRP MCA4764P E P WB 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Adalimumab HCA204 E 0.1 mg loader
Human anti Adalimumab:HRP HCA204P E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C* E 0.2 mg loader
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml loader

Product Specific References

References for Adalimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-190.
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