Adalimumab antibody | AbD18655_hIgG1

Human anti Adalimumab:HRP

Product Type
Monoclonal Antibody
Clone
AbD18655_hIgG1
Isotype
IgG1
Specificity
Adalimumab

Product Code Applications Pack Size List Price Your Price Qty
HCA204P
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
HCA204
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
HCA204G
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SDS Safety Datasheet SDS
E 1 mg loader
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loader
Search for Batch Specific Datasheets

Human Anti-Adalimumab, clone AbD18655_hIgG1, is an ultra-high affinity anti-idiotypic antibody that specifically recognizes adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. Clone AbD18655_hIgG1 can be used to measure the levels of adalimumab and biosimilar products using bioanalytical assays. It may also be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient serum.

Adalimumab (branded as Humira) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all Anti-Adalimumab Antibodies.

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Adalimumab
Affinity
The monovalent intrinsic affinity of this antibody in an unconjugated format was measured as KD=0.06 nM by real time, label-free molecular interaction analysis on immobilized adalimumab.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Humira is a registered trademark of Abbott Laboratories.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a sandwich ELISA together with HCA202 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Human anti Adalimumab HCA202 E 0.1 mg loader
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loader
Description Human anti Adalimumab
Human anti Adalimumab HCA203 E 0.1 mg loader
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loader
Description Human anti Adalimumab
Human anti Adalimumab HCA205 E 0.1 mg loader
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loader
Description Human anti Adalimumab
Human anti Adalimumab (Drug/Target Complex) HCA207 E 0.1 mg loader
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loader
Description Human anti Adalimumab (Drug/Target Complex)
Recombinant Human TNF Alpha PHP051 E FN WB 50 µg loader
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loader
Description Recombinant Human TNF Alpha

References for Adalimumab antibody

  1. Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
    MAbs. 11 (1): 178-190.

Synonyms
Humira
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Adalimumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"