Adalimumab antibody | AbD18664_hIgG1

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Anti Adalimumab Antibody, clone AbD18664_hIgG1 thumbnail image 1

Anti Adalimumab Antibody, clone AbD18664_hIgG1 thumbnail image 2

Anti Adalimumab Antibody, clone AbD18664_hIgG1 thumbnail image 3

Anti Adalimumab Antibody, clone AbD18664_hIgG1 gallery image 1 — **HCA205** specificity ELISA using various antigens as coating reagents. HRP conjugated Human anti-Adalimumab antibody (**HCA205**) was used as the detection reagent.

Anti Adalimumab Antibody, clone AbD18664_hIgG1 gallery image 2 — HCA205 Anti-Drug-Antibody (ADA) assay, bridging format. Adalimumab was used as the coating reagent followed by anti-Adalimumab antibodies in human IgG1 format (black squares HCA203; red circles HCA204; green triangles **HCA205**). Detection was performed with HRP conjugated Adalimumab.

Anti Adalimumab Antibody, clone AbD18664_hIgG1 gallery image 3 — Inhibition of Adalimumab binding to TNFα by **HCA205**. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (**HCA205**) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using HRP conjugated HCA207 (Human anti Adalimumab-TNFα complex specific).

Human anti Adalimumab

Anti-adalimumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human immunoglobulin format; it is suitable as a reference standard in an immune response assay to measure anti-drug antibody levels for adalimumab or biosimilars.

Product Type: Monoclonal Antibody
Clone: AbD18664_hIgG1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
HCA205	E			0.1 mg

Summary
Useful Reagents

Human anti Adalimumab, clone AbD18664_hIgG1, is an anti-idiotypic antibody that specifically recognizes the adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. Clone AbD18664_hIgG1 can be used to measure the levels of adalimumab and biosimilar products using bioanalytical assays.

Adalimumab (branded as Humira^®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

View a summary of all anti-adalimumab antibodies.

Product Details

Product Form: Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: Adalimumab
Affinity: The monovalent intrinsic affinity of this antibody was measured as K_D=10.5 nM by real time, label-free molecular interaction analysis on immobilized adalimumab.
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.

More Information

Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Humira® is a registered trademark of Abbott Laboratories.
Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory: For research purposes only

Applications of Adalimumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA: This product may be used in an indirect ELISA or as a reference standard in immune response assays.
Protocol: ADA bridging ELISA

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Hispec Assay Diluent	BUF049A	50 ml	E IY
Human anti Adalimumab	HCA202	0.1 mg	E
Human anti Adalimumab	HCA203	0.1 mg	E
Human anti Adalimumab	HCA204	0.1 mg	E
Human anti Adalimumab:HRP	HCA204P	0.1 mg	E
Human anti Adalimumab (Drug/Target Complex)	HCA207	0.1 mg	E
LYNX Rapid HRP Antibody Conjugation Kit	LNK002P	3 Conjugations For 400µg Antibody	CJ
Mouse anti Human IgG (Fc) CH2 Domain:HRP	MCA647P	0.2 mg	C* E
Recombinant Human TNF Alpha	PHP051	50 µg	E FN WB

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