Tocilizumab antibody | AbD22155_hIgG1

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Human anti Tocilizumab:HRP

Anti-tocilizumab antibody is a non-neutralizing anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assays for tocilizumab and biosimilars. It does not inhibit the binding of tocilizumab to IL-6R and therefore is ideal for a PK bridging ELISA with HCA252 to detect total drug.

Human anti Tocilizumab

Anti-tocilizumab antibody is a non-neutralizing anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assays for tocilizumab and biosimilars. It does not inhibit the binding of tocilizumab to IL-6R and therefore is ideal for a PK bridging ELISA with HCA252 to detect total drug.

Product Type
Monoclonal Antibody
Clone
AbD22155_hIgG1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
HCA257P
Datasheet
E
SDS
0.1 mg loader

HCA257
Datasheet
E
SDS
0.1 mg loader

Human Anti-Tocilizumab Antibody, clone AbD22155_hIgG1 is a non-paratope specific, anti-idiotypic antibody that binds to free tocilizumab and also to tocilizumab bound to its target, human interleukin-6 receptor (IL-6R). The non-inhibitory antibody can be used to measure total levels of tocilizumab and biosimilar products in bioanalytical assays.

Clone AbD22155_hIgG1 can be used to develop a pharmacokinetic (PK) bridging assay to measure total drug levels (free drug, partially bound and fully bound). This non-inhibitory antibody is recommended as the detection antibody, paired with the inhibitory capture antibody clone AbD21362 (HCA252). This combination enables measurement of free and partially bound tocilizumab, which is equivalent to total drug when trough serum levels are above 10 µg/ml.

Tocilizumab (RoActemra/Actemra) is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.

View a summary of all anti-tocilizumab antibodies

Product Details

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Tocilizumab and tocilizumab-sIL-6R complex
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD = 31 nM by real time, label free molecular interaction analysis on immobilized tocilizumab.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Tocilizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug
ELISA
This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Tocilizumab HCA252 E* 0.1 mg loader
Human anti Tocilizumab HCA253 E 0.1 mg loader
Human anti Tocilizumab HCA254 E 0.1 mg loader
Human anti Tocilizumab HCA255 E 0.1 mg loader
Human anti Tocilizumab HCA256 E 0.1 mg loader
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Tocilizumab HCA252 E* 0.1 mg loader
Human anti Tocilizumab HCA253 E 0.1 mg loader
Human anti Tocilizumab HCA254 E 0.1 mg loader
Human anti Tocilizumab HCA255 E 0.1 mg loader
Human anti Tocilizumab HCA256 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK003P CJ 1 Conjugation For 4mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK004P CJ 5 Conjugations For 4mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK005P CJ 1 Conjugation For 20mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK006P CJ 3 Conjugations For 40µg Antibody

Product Specific References

References for Tocilizumab antibody

  1. Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
    US Patent Application US20170315118A1

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