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Protocol: ADA Bridging ELISA for Use with Anti-Tocilizumab Antibodies
Anti-Drug Antibody (ADA) Bridging ELISA: for Use with Anti-Tocilizumab Monoclonal Antibodies HCA253, HCA254, HCA255 and HCA256
This method provides a procedure for generating an ADA ELISA standard curve with an Anti-Tocilizumab Antibody, catalog number HCA253, HCA254, HCA255 or HCA256 using tocilizumab antibody for capture and detection. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms