Tocilizumab antibody | AbD21346_hIgG1
A microtiter plate was coated over night with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti tocilizumab (clone AbD21346_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtiter plate was coated over night with human IgG1/kappa, human IgG1/lambda or tocilizumab at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti tocilizumab (clone AbD21346_hIgG1) titrated to the given concentrations in PBST, followed by QuantaBlu™ fluorogenic peroxidase substrate. Data is presented as the mean of three measurements
A microtiter plate was coated over night with Human anti tocilizumab (clone AbD21362, HCA252) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of tocilizumab. Detection was performed using HRP-conjugated Human anti tocilizumab (clone AbD21346_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is presented as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtitre plate was coated over night with tocilizumab at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of different Human anti tocilizumab clones, AbD21338_hIgG1 (HCA253), AbD21364_hIgG1 (HCA254), AbD21346_hIgG1 (HCA255) and AbD21345_hIgG1 (HCA256). Detection was performed using HRP-conjugated tocilizumab. Data is presented as the mean of two measurements. HRP-conjugation of tocilizumab used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtiter plate was coated over night with human interleukin 6 receptor at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of tocilizumab (0.3 μg/ml) plus increasing concentrations of the monovalent Fab fragment of the inhibitory Human anti tocilizumab clone AbD21346_hIgG (HCA255) or the non-inhibitory clone AbD22155_hIgG1 (HCA257) was added. Free tocilizumab, still capable of binding to the human interleukin 6 receptor-coated plate, was detected using Mouse anti Human IgG (Fc) CH2 Domain:HRP (MCA647P) and QuantaBlu™ fluorgenic peroxidase substrate. Data is presented as the mean of three measurements
Human anti Tocilizumab
Anti-tocilizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human IgG1 format, suitable for bioanalytical assays for tocilizumab and biosimilars. It is recommended as a reference standard with medium affinity for an ADA bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD21346_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| HCA255 | E | 0.1 mg |
Human anti tocilizumab, clone AbD21346_hIgG1 is a Type 1 anti-idiotypic antibody that specifically recognizes free tocilizumab but not free human Interleukin-6 Receptor (IL-6R) or the drug/IL-6R complex. Clone AbD21346_hIgG1 inhibits the binding of tocilizumab to IL-6R and can be used to measure free tocilizumab levels in serum from patients. This clone can also be used for pharmacokinetic (PK) assay development or in the development and calibration of anti-drug antibody (ADA) assays.
Tocilizumab, marketed under the brand name RoActemra® in Europe and Actemra® in North America, is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. This therapeutic antibody, directed against IL-6R, acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.
Clone AbD21346_hIgG1 is one of a range of Type 1 (HCA252), (HCA253), (HCA254), (HCA256) and Type 2 (HCA257) anti-idiotypic antibodies to tocilizumab available from Bio-Rad and forms part of a wider and expanding portfolio of anti-idiotypic antibodies that have been developed for the detection of biotherapeutics.
Tocilizumab, marketed under the brand name RoActemra® in Europe and Actemra® in North America, is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. This therapeutic antibody, directed against IL-6R, acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.
Clone AbD21346_hIgG1 is one of a range of Type 1 (HCA252), (HCA253), (HCA254), (HCA256) and Type 2 (HCA257) anti-idiotypic antibodies to tocilizumab available from Bio-Rad and forms part of a wider and expanding portfolio of anti-idiotypic antibodies that have been developed for the detection of biotherapeutics.
Product Details
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Tocilizumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=6.2 nM by real time, label-free molecular interaction analysis on immobilized tocilizuimab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Shelf Life
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Tocilizumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay.
Protocol: ADA bridging ELISA
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human anti Tocilizumab | HCA252 | 0.1 mg | E* | ||
| Human anti Tocilizumab | HCA253 | 0.1 mg | E | ||
| Human anti Tocilizumab | HCA254 | 0.1 mg | E | ||
| Human anti Tocilizumab | HCA256 | 0.1 mg | E | ||
| Human anti Tocilizumab | HCA257 | 0.1 mg | E | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | 1 Conjugation For 400µg Antibody | CJ | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | 3 Conjugations For 400µg Antibody | CJ | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK003P | 1 Conjugation For 4mg Antibody | CJ | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK004P | 5 Conjugations For 4mg Antibody | CJ | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK005P | 1 Conjugation For 20mg Antibody | CJ | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK006P | 3 Conjugations For 40µg Antibody | CJ |
