Tocilizumab antibody | AbD21362

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Human anti Tocilizumab

Anti-tocilizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development to measure levels of tocilizumab or biosimilars. It is recommended as capture antibody in PK bridging ELISA with HCA253/HCA253P.

Product Type
Monoclonal Antibody
Clone
AbD21362
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA252 E* 0.1 mg
Human anti Tocilizumab, clone AbD21362 is a paratope specific, anti-idiotypic antibody that binds to free tocilizumab but not to human Interleukin-6 Receptor (IL-6R) or the drug/IL-6R complex. The antibody can be used to measure the levels of tocilizumab and biosimilar products in bioanalytical assays.

Clone AbD21362 can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody is recommended as the capture antibody, paired with the detection antibody clone AbD21338_hIgG HRP-conjugated and in full immunoglobulin (Ig) format (HCA253).

Tocilizumab, marketed under the brand name RoActemra® in Europe and Actemra® in North America, is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.

View a summary of all anti-tocilizumab antibodies

Product Details

Product Form
A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Tocilizumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.9 nM by real time, label-free molecular interaction analysis on immobilized tocilizuimab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Tocilizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1
  1. 1 NB. The Rat anti DYKDDDDK-tag antibody, clone 6F7 is not suitable for use with this antibody as clone 6F7 exhibits cross reactivity with the human IL-6R
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct or indirect ELISA or as a capture reagent in a PK bridging ELISA together with HCA253 as the detection reagent.
Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Tocilizumab HCA253 0.1 mg E
Human anti Tocilizumab HCA254 0.1 mg E
Human anti Tocilizumab HCA255 0.1 mg E
Human anti Tocilizumab HCA256 0.1 mg E
Human anti Tocilizumab HCA257 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK001P 1 Conjugation For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK003P 1 Conjugation For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK004P 5 Conjugations For 4mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK005P 1 Conjugation For 20mg Antibody CJ
LYNX Rapid HRP Antibody Conjugation Kit LNK006P 3 Conjugations For 40µg Antibody CJ