Tocilizumab antibody | AbD22155_hIgG1




Human anti Tocilizumab:HRP
Anti-tocilizumab antibody is a non-neutralizing anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assays for tocilizumab and biosimilars. It does not inhibit the binding of tocilizumab to IL-6R and therefore is ideal for a PK bridging ELISA with HCA252 to detect total drug.
Human anti Tocilizumab
Anti-tocilizumab antibody is a non-neutralizing anti-idiotypic antibody in full IgG1 format, ideal for bioanalytical assays for tocilizumab and biosimilars. It does not inhibit the binding of tocilizumab to IL-6R and therefore is ideal for a PK bridging ELISA with HCA252 to detect total drug.
- Product Type
- Monoclonal Antibody
- Clone
- AbD22155_hIgG1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA257P | E | 0.1 mg |
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HCA257 | E | 0.1 mg |
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Product Details
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
- 0.01% Thiomersal
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD = 31 nM by real time, label free molecular interaction analysis on immobilized tocilizumab.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd. - Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Tocilizumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug - ELISA
- This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure total drug
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Tocilizumab | HCA252 | E* | 0.1 mg |
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Human anti Tocilizumab | HCA253 | E | 0.1 mg |
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Human anti Tocilizumab | HCA254 | E | 0.1 mg |
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Human anti Tocilizumab | HCA255 | E | 0.1 mg |
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Human anti Tocilizumab | HCA256 | E | 0.1 mg |
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Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Tocilizumab | HCA252 | E* | 0.1 mg |
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Human anti Tocilizumab | HCA253 | E | 0.1 mg |
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Human anti Tocilizumab | HCA254 | E | 0.1 mg |
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Human anti Tocilizumab | HCA255 | E | 0.1 mg |
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Human anti Tocilizumab | HCA256 | E | 0.1 mg |
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LYNX Rapid HRP Antibody Conjugation Kit | LNK001P | CJ | 1 Conjugation For 400µg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK003P | CJ | 1 Conjugation For 4mg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK004P | CJ | 5 Conjugations For 4mg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK005P | CJ | 1 Conjugation For 20mg Antibody | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK006P | CJ | 3 Conjugations For 40µg Antibody |
Product Specific References
References for Tocilizumab antibody
-
Kashiwagi, N. et al. (2017) Method for measuring anti-drug antibody
US Patent Application US20170315118A1
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