Tocilizumab antibody | AbD21338_hIgG1

Human anti Tocilizumab:HRP

Product Type
Monoclonal Antibody
Clone
AbD21338_hIgG1
Isotype
IgG1
Specificity
Tocilizumab

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HCA253P
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Human Anti-Tocilizumab Antibody, clone AbD21338 is a paratope specific, anti-idiotypic antibody that binds to free tocilizumab but not to human Interleukin-6 Receptor (IL-6R) or the drug/IL-6R complex. The antibody can be used to measure the levels of tocilizumab and biosimilar products in bioanalytical and patient monitoring assays.

Clone AbD21338_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator (high affinity) in an anti-drug antibody (ADA) assay. Additionally it can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody is recommended as the detection antibody, paired with Anti-Tocilizumab Antibody clone AbD21362 (HCA252) in monovalent Fab format as the capture antibody.

Tocilizumab (RoActemra/Actemra) is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.

View a summary of all anti-tocilizumab antibodies

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Tocilizumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=0.1 nM by real time, label-free molecular interaction analysis on immobilized tocilizuimab.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Tocilizumab HCA252 E* 0.1 mg loader
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Description Human anti Tocilizumab
Human anti Tocilizumab HCA254 E 0.1 mg loader
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Description Human anti Tocilizumab
Human anti Tocilizumab HCA255 E 0.1 mg loader
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Description Human anti Tocilizumab
Human anti Tocilizumab HCA256 E 0.1 mg loader
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Description Human anti Tocilizumab
Human anti Tocilizumab HCA257 E 0.1 mg loader
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Description Human anti Tocilizumab

Synonyms
ACTEMRA®
ROACTEMRA®
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"