Tocilizumab antibody | AbD21364_hIgG1

100% Secure


Human anti Tocilizumab

Anti-tocilizumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human IgG1 format, suitable for bioanalytical assays for tocilizumab and biosimilars. It is recommended as a reference standard with high affinity for an ADA bridging ELISA.

Product Type
Monoclonal Antibody
Clone
AbD21364_hIgG1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
HCA254
Datasheet
E
MSDS
0.1 mg loader

Human Anti-Tocilizumab Antibody, clone AbD21364_hIgG1 is a paratope specific, anti-idiotypic antibody that binds to free tocilizumab but not to human Interleukin-6 Receptor (IL-6R) or the drug/IL-6R complex.

Clone AbD21364_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator (high affinity) in an anti-drug antibody (ADA) assay.

Tocilizumab (RoActemra/Actemra) is a humanized IgG1/kappa monoclonal antibody approved for the treatment of moderate to severe rheumatoid arthritis and for the treatment of systemic juvenile idiopathic arthritis. The therapeutic antibody is directed against IL-6R and acts by blocking the binding of IL-6R (both the soluble form, sIL-6R, and the membrane bound form, mIL-6R) to IL-6, thus impeding the pro-inflammatory properties of this cytokine.

View a summary of all anti-tocilizumab antibodies

Product Details

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Tocilizumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD = 0.3 nM by real time, label free molecular interaction analysis on immobilized tocilizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Actemra® and RoActemra® are registered trademarks of Hoffman-La Roche and Chugai Pharmaceuticals co., Ltd.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad
Regulatory
For research purposes only

Applications of Tocilizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA or as a detection reagent in a bridging ELISA together with HCA252 as the capture reagent.
Protocol: PK bridging ELISA to measure free drug
This antibody is fully human and can be used as a reference standard in an ADA assay.
Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Tocilizumab HCA252 E* 0.1 mg loader
Human anti Tocilizumab HCA253 E 0.1 mg loader
Human anti Tocilizumab HCA255 E 0.1 mg loader
Human anti Tocilizumab HCA256 E 0.1 mg loader
Human anti Tocilizumab HCA257 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK001P CJ 1 Conjugation For 400µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK003P CJ 1 Conjugation For 4mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK004P CJ 5 Conjugations For 4mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK005P CJ 1 Conjugation For 20mg Antibody
LYNX Rapid HRP Antibody Conjugation Kit LNK006P CJ 3 Conjugations For 40µg Antibody