Recommended Antibody Protocols
Overview
Whether you are performing flow cytometry, ELISA, western blotting, immunofluorescence, or cell-based assays, following well-established protocols from a trusted source can help improve consistency, reduce experimental variability, and generate reliable data.
Bio-Rad has compiled a collection of recommended protocols designed to support researchers at every stage of their workflow, from sample preparation and staining through to data acquisition and analysis.
alamarBlue Protocols
Detailed guides to help you use alamarBlue
- QC test for alamarBlue
- Method to determine LD50 using alamarBlue
- General method for measuring cytotoxicity or proliferation using alamarBlue (spectrophotometry method and fluorescence method)
- Method for determining optimum length of incubation and plating density
- Spectrophotometry calculations for using different filters
- alamarBlue example experiments
View All alamarBlue Protocols
MCM2 Protocol
Detailed guide to help you use MCM2
ELISA Protocols
General ELISA protocols
- Indirect
- Competition (Inhibition)
- Direct ELISA with streptavidin-biotin detection
- Sandwich ELISA with streptavidin-biotin detection
- Sandwich ELISA with direct detection
Protocols for use with anti-biotherapeutic antibodies
- PK bridging ELISA measuring free drug
- PK bridging ELISA measuring total drug
- PK antigen capture ELISA measuring bound drug exclusively
- ADA bridging ELISA
View All ELISA Protocols
Flow Cytometry (FC) Protocols
- Preparation of tissue culture cell lines for flow cytometry
- Preparation of peripheral blood mononuclear cells for flow cytometry
- Preparation of peritoneal macrophages, bone marrow, thymus, and spleen cells for flow cytometry
- Direct immunofluorescence staining of surface epitopes of cells and whole blood
- Indirect immunofluorescence staining of surface epitopes of cells and whole blood
- Direct immunofluorescence staining of immunoglobulin light chains on B lymphocytes in whole blood
- Direct immunofluorescence staining of intracellular antigens: the Leucoperm method
- Direct immunofluorescence staining of intracellular antigens: the methanol plus Leucoperm method
- Direct immunofluorescence staining of intracellular cytokines in whole blood
- Direct immunofluorescence staining of intracellular antigens: the digitonin method
- Direct immunofluorescence staining of intracellular antigens: the paraformaldehyde/saponin method
- Propidium iodide staining of cells for cell cycle analysis
- Propidium iodide staining of cells for cell cycle analysis in combination with direct immunofluorescence intracellular staining
- BrdU staining of cells for cell cycle analysis
- Unprimed T-cell activation: pharmacologic methods
- Unprimed T-cell activation: antibody stimulation method
- Primed T-cell activation: antigen-presenting cell co-culture method
- Measuring cell proliferation using CytoTack, a cell-permeable dye
- Direct immunofluorescence staining of intracellular antigens using antibodies labeled with StarBright™ Dyes
- Direct immunofluorescence staining of whole blood for intracellular CD68: the Leucoperm method
- Direct immunofluorescence staining of cells with StarBright Dye-conjugated antibodies
View All Flow Cytometry Protocols
Immunofluorescence (IF) Protocols
Immunohistochemistry (IHC) Protocols
Protocols for IHC-frozen and IHC-paraffin
- Indirect Immunostaining of frozen tissue sections
- Streptavidin-biotin immunostaining of frozen tissue sections
- PAP/APAAP Immunostaining of frozen tissue sections
- Indirect immunostaining of paraffin-embedded tissue sections
- Streptavidin-biotin immunostaining of paraffin-embedded tissue sections
- PAP/APAAP immunostaining of paraffin embedded tissue sections
- Antigen retrieval techniques for use with formalin-fixed paraffin-embedded sections
- Antigen retrieval techniques: For use with F4/80 immunostaining of paraffin-embedded sections
- Fixative Recipes
Western Blot (WB) Protocols
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