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Direct Immuno-fluorescence Staining of Immunoglobulin Light Chains on B Lymphocytes in Whole Blood by Flow Cytometry
For use with Bio-Rad’s directly-conjugated dual colour reagents recognising human kappa and lambda immunoglobulin light chains.
The immunofluorescent staining of immunoglobulin expression by B lymphocytes in whole blood requires a procedure to remove serum immunoglobulin (which otherwise interfere and block staining with immunoglobulin-specific antibodies).
This method provides a general procedure for use with the majority of Bio-Rad reagents. In some cases specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with the product and batch specific information provided with each vial. A certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques; these are guidelines only and may need to be adjusted for particular applications.
Reynolds, W.M. et al. (1992) A simple technique for the determination of k and l immunoglobulin light chain expression by B cells in whole blood. J. Imm. Meths. 151:123-129.