Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Bio-Rad-Antibodies.com relies on third-party cookies to show you pricing, allow you to order online, and connect you to My Bio-Rad. Please amend your browser settings to enable third-party cookies and access this website’s full functionality.
s
A. Lysine HCl solution
Lysine HCl, 13.7 g
Na2HPO4.2H2O (Sorenson’s salt), 1.8g
Distilled water, 475 ml
Dissolve lysine HCl in 375 ml of distilled water.
Dissolve Sorenson’s salt in 100 ml of distilled water.
Add Sorenson’s salt solution to lysine solution until pH 7.4 is reached.
Discard any remaining Sorenson’s salt solution.
B. Paraformaldehyde solution
Paraformaldehyde, 2.5 g
Distilled water, 200 ml
1 M NaOH, few drops
Heat the distilled water (or, sometimes a 5% dextrose solution is used) to 60°C.
Add the paraformaldehyde, and a few drops of 1 M NaOH while stirring to clear the solution.
Leave to cool (refrigerate overnight if necessary).
C. Final PLP solution
Lysine HCl solution (Solution A)
Paraformaldehyde solution (Solution B)
NaIO4 (Sodium Periodate), 2.14 g
Make up the final solution immediately before use by mixing together the Lysine HCl solution and, the paraformaldehyde solution, and then adding 2.14 g of NaIO4.
Make up to 1 liter with 0.1 M phosphate buffer and, adjust the pH to 7.2.
Based on: McLean, I.W. and Nakane, P.K. (1974). Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J. Histochem. Cytochem. 22:1077-1083.
Paraformaldehyde (from 16% stock solution), 125 ml
Picric acid (saturated aqueous), 150 ml
10 M NaOH, a few drops
Combine paraformaldehyde and picric acid solution. Make up to 1 liter with 0.1 M phosphate buffer. Adjust to pH 7.3 using 10 M NaOH.
Based on: Stefanini, M. et al. (1967). Fixation of ejaculated spermatozoa for electron microscopy. Nature 216: 173-174.
Picric acid (saturated aqueous), 75 ml
Formalin (37-40% formaldehyde), 25 ml
Glacial acetic acid, 5 ml
Combine all three to make up the fixative.
Based on: Hopwood, D. (1990). Fixation and fixatives. In Bancroft, J.D. et al. (eds). Theory and practice of histological techniques. Churchill Livingstone, New York, p21-42.