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Dissolve lysine HCl in 375 ml of distilled water.
Dissolve Sorenson’s salt in 100 ml of distilled water.
Add Sorenson’s salt solution to lysine solution until pH 7.4 is reached.
Discard any remaining Sorenson’s salt solution.
Heat the distilled water (or, sometimes a 5% dextrose solution is used) to 60°C.
Add the paraformaldehyde, and a few drops of 1 M NaOH while stirring to clear the solution.
Leave to cool (refrigerate overnight if necessary).
Make up the final solution immediately before use by mixing together the Lysine HCl solution and, the paraformaldehyde solution, and then adding 2.14 g of NaIO4.
Make up to 1 liter with 0.1 M phosphate buffer and, adjust the pH to 7.2.
Based on: McLean, I.W. and Nakane, P.K. (1974). Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J. Histochem. Cytochem. 22:1077-1083.
Combine paraformaldehyde and picric acid solution. Make up to 1 liter with 0.1 M phosphate buffer. Adjust to pH 7.3 using 10 M NaOH.
Based on: Stefanini, M. et al. (1967). Fixation of ejaculated spermatozoa for electron microscopy. Nature 216: 173-174.
Combine all three to make up the fixative.
Based on: Hopwood, D. (1990). Fixation and fixatives. In Bancroft, J.D. et al. (eds). Theory and practice of histological techniques. Churchill Livingstone, New York, p21-42.