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This quality control (QC) method can be used to determine the standard absorbance values for alamarBlue under selected experimental conditions. This control is designed to be used for investigations into how values are affected by type of plate and the chosen media.
|Wavelength (nm)||Average Absorbance (Standard Deviation)|
N.B. Absorbance values may be affected by the type of plate (whether round or flat bottom) and the plate manufacturer (See Appendix 4 in the technical datasheet for further information about the effect of plates on absorbance).
No QC protocol is recommended for fluorescence, since fluorescence units are arbitrary and the scale used varies widely from one instrument to another.
For an indication of the absorbance and fluorescence values for the fully oxidized and reduced forms of alamarBlue with different media, please visit Frequently Asked Questions.
Although it is preferable to use the recommended wavelengths, a correction factor can be calculated allowing the use of one alternative filter.
570 nm and 600 nm are the recommended wavelengths; only one wavelength may be altered for its respective alternative wavelength.