Direct staining of intracellular CD68 by flow cytometry: Leucoperm Accessory Reagent method
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Method for cell permeabilization required prior to intracellular staining using Leucoperm Accessory Reagent.
The method described below produces excellent results in our labs; however, other permeabilization techniques have been published, and may also be used successfully for this application. These methods should always be used in conjunction with the product and batch specific information provided with each vial. A certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques; these are guidelines only and may need to be adjusted for particular applications.
Optional: 0.5% (w/v) paraformaldehyde in PBS (Note: dissolve on heated stirrer and cool before use).
The following should be considered when designing your flow cytometry experiments: