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Antigen Retrieval Techniques: For use with F4/80 Immunostaining of Paraffin-Embedded Sections
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Antigen unmasking procedures are recommended to achieve optimum IHC staining for F4/80, as the F4/80 antigen can be masked by prolonged formalin-fixation and the paraffin-embedding process.
This procedure is recommended for tissues fixed for 24 hours in Neutral Buffered Formalin (NBF).
A number of buffers may be used for this purpose; the precise choice is usually recommended on the product specific datasheet or determined experimentally. For your convenience Bio-Rad offers a range of pre-prepared Antigen Retrieval Buffer at different pH levels guaranteeing consistency of performance and reliability.
Alternatively, buffers may be prepared using the recipes listed below. For further details contact Technical Support.
This procedure is recommended for tissues fixed for 7 days or more in Neutral Buffered Formalin (NBF)
Benefits of using this antigen retrieval procedure can be seen below. Image A. shows IHC staining with no pre-treatment and image B. shows IHC staining that can be achieved after pre-treatment, clearly resulting in superior staining.
Image A. F4/80 antibody staining of liver tissue fixed in NBF for 28 days, no antigen retrieval
Image B. F4/80 antibody staining of liver tissue fixed in NBF for 28 days with citrate buffer & heat-mediated antigen retrieval
Our F4/80 antibody (Clone Cl: A3-1) (MCA497) is a well characterized and highly cited F480 antibody. Research by Citeab recently showed that it was the most cited physiology antibody, with 155 citations (at the time of publishing).