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This is a general procedure for use with the majority of Bio-Rad reagents recommended for direct ELISA with streptavidin- biotin detection. In some cases specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with product and batch specific information available for each vial. Please note that a certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques - these are guidelines only and may need to be adjusted for particular applications.