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Direct Immunofluorescence Staining of Cells with StarBright Dyes
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This method provides a general procedure for use with StarBright Dyes, for staining cells in tubes or a 96-well plate. In some cases, specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with the product and batch specific information provided with each vial.
A certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques; these are guidelines only and may need to be adjusted for particular applications or cell types.
The following should be considered when designing your flow cytometry experiments.
Appropriate controls should always be carried out. This may include unstained cells to help instrument setup and autofluorescence detection, and a positive and negative control. For multicolor staining, we recommend using a fluorescence minus one control, which shows the influence of other fluorophores in the missing channel and helps set gating boundaries.