Measuring Cell Proliferation Using Cell Permeable Dyes
This method provides a general procedure for staining peripheral blood mononuclear cells with cell permeable dyes, prior to inclusion in activation and proliferation protocols such as FC15, FC16 and FC17. All blood samples must be collected into heparin anticoagulant as EDTA will interfere with the cell stimulation process.
This protocol is for use with the majority of Bio-Rad reagents. In some cases specific recommendations are provided on product datasheets, and these methods should always be used in conjunction with the product and batch specific information provided with each vial. A certain level of technical skill and immunological knowledge is required for the successful design and implementation of these techniques; these are guidelines only and may need to be adjusted for particular applications.
Appropriate controls should be carried out for flow cytometry, consider including the following: