alamarBlue can be used for applications such as functional assay, immunofluorescence, and ELISA.
This reagent is offered in two sizes:
25 ml = Enough for 2,500 wells
100 ml = Enough for 10,000 wells
Note: Calculations assume 100 µl final volume per well (96-well plate).
Detailed protocols to help you use alamarBlue
alamarBlue has been extensively referenced and used in a wide range of research areas. Below is a list of protocols including quality control, experimental optimization, general methods, examples, and calculations for using alamarBlue.
Detailed protocols for use of alamarBlue:
alamarBlue Tips & Advice
QC test for alamarBlue
Method to determine LD50 using alamarBlue
General method for measuring cytotoxicity or proliferation using alamarBlue (spectrophotometry method and fluorescence method)
Method for determining optimum length of incubation and plating density
Spectrophotometry calculations for using different filters
alamarBlue example experiments